Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil

• Published in: Environmental science and pollution research international Vol. 21; no. 15; pp. 9385 - 9395
• Main Authors: Pacwa-Płociniczak, Magdalena, Płaza, Grażyna Anna, Poliwoda, Anna, Piotrowska-Seget, Zofia
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 2014; Springer Berlin Heidelberg; Springer Nature B.V
• Subjects: Agar; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Bacteria; Biodegradation, Environmental; Bioremediation; biosurfactants; Biosynthesis; blood; Crude oil; culture media; Degradation; DNA polymerase; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental Pollution; Environmental Restoration and Remediation - methods; Enzymes; Fourier transforms; Gene amplification; genes; Heavy metals; Hexadecane; Hydrocarbons; Hydrocarbons - analysis; Hydrocarbons - metabolism; Methylene blue; Microorganisms; Naphthalene; NMR; Nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; Oil; Oil spills; oils; Petroleum; Petroleum - analysis; Petroleum - metabolism; Petroleum hydrocarbons; Phenanthrene; Pseudomonas; Pseudomonas - metabolism; Research Article; Rhamnolipids; soil; Soil (material); Soil - chemistry; Soil contaminants; Soil contamination; Soil Pollutants - analysis; Soil Pollutants - metabolism; Soil pollution; Strain; Studies; Surface-Active Agents - analysis; Surface-Active Agents - metabolism; Surfactants; Syrups & sweeteners; Waste Water Technology; Water Management; Water Pollution Control; Poland; Rhamnolipid; Crude oil; gene; sp. P-1; Hydrocarbon degradation; Biosurfactant production
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-014-2872-1

The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13 % of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. ¹H and ¹³C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.