Manipulating circadian clock neuron firing rate resets molecular circadian rhythms and behavior
Using optogenetic manipulations and bioluminescence imaging of suprachiasmatic nucleus (SCN) firing rate, this study examines the interaction between molecular, electrical and behavioral circadian rhythms in mice. The study shows that alteration of clock neuron firing can reset molecular and behavio...
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Published in | Nature neuroscience Vol. 18; no. 3; pp. 373 - 375 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
New York
Nature Publishing Group US
01.03.2015
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Using optogenetic manipulations and bioluminescence imaging of suprachiasmatic nucleus (SCN) firing rate, this study examines the interaction between molecular, electrical and behavioral circadian rhythms in mice. The study shows that alteration of clock neuron firing can reset molecular and behavioral circadian rhythms, and this effect required neuronal network interaction within the SCN. Thus, clock neuron spiking is fundamental to circadian pacemaking as both an input to and output of the neuronal network responsible for circadian behavior.
To examine the interaction between molecular, electrical and behavioral circadian rhythms, we combined optogenetic manipulation of suprachiasmatic nucleus (SCN) firing rate with bioluminescence imaging and locomotor activity monitoring. Manipulating firing rate reset circadian rhythms both
ex vivo
and
in vivo
, and this resetting required spikes and network communication. This suggests that SCN firing rate is fundamental to circadian pacemaking as both an input to and output of the molecular clockworks. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1097-6256 1546-1726 |
DOI: | 10.1038/nn.3937 |