An intra-tumoral niche maintains and differentiates stem-like CD8 T cells
Tumour-infiltrating lymphocytes are associated with a survival benefit in several tumour types and with the response to immunotherapy . However, the reason some tumours have high CD8 T cell infiltration while others do not remains unclear. Here we investigate the requirements for maintaining a CD8 T...
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Published in | Nature (London) Vol. 576; no. 7787; pp. 465 - 470 |
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Main Authors | , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Nature Publishing Group
01.12.2019
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Subjects | |
Online Access | Get full text |
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Summary: | Tumour-infiltrating lymphocytes are associated with a survival benefit in several tumour types and with the response to immunotherapy
. However, the reason some tumours have high CD8 T cell infiltration while others do not remains unclear. Here we investigate the requirements for maintaining a CD8 T cell response against human cancer. We find that CD8 T cells within tumours consist of distinct populations of terminally differentiated and stem-like cells. On proliferation, stem-like CD8 T cells give rise to more terminally differentiated, effector-molecule-expressing daughter cells. For many T cells to infiltrate the tumour, it is critical that this effector differentiation process occur. In addition, we show that these stem-like T cells reside in dense antigen-presenting-cell niches within the tumour, and that tumours that fail to form these structures are not extensively infiltrated by T cells. Patients with progressive disease lack these immune niches, suggesting that niche breakdown may be a key mechanism of immune escape. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions C.S.J. and H.K. conceived and designed the study and composed the manuscript. C.S.J., V.A.M., M.G.S., C.P.F., M.A. and H.K. designed experiments. C.S.J., N.P., J.W.C., M.C., R.M.V., W.H.H., D.M., K.M., A.I.K., K.K., Y.M.C., A.K., A.O.K., A.R. and H.K. collected flow cytometry data. C.S.J., N.P., M.C. and H.K. analysed flow cytometry data. C.S.J., N.P., M.C., A.R. and H.K. performed fluorescence activated cell sorting. C.S.J., N.P. and M.C. performed RNA and DNA extractions. C.S.J., S.W., R.L. and A.G.S. optimized and performed immunofluorescence slide scanning. C.S.J. and H.K. developed quantitative immunofluorescence techniques and performed quantitative analysis of immunofluorescence data. W.H.H., D.M. and H.K. performed RNA sequencing analysis. N.P., M.C., K.K., Y.M.C., A.K. and H.K. performed in vitro T cell assays. N.P. and H.K. performed whole-genome methylation analysis. R.A., S.J.I. and A.O.K. provided critical expertise and contributed specific analysis. V.A.M., M.G.S., C.P.F. and M.A. provided clinical samples. C.S.J., J.W.C. and A.R. collected and organized clinical data. K.M., A.O.O., P.M. and C.E. provided annotation and scoring of pathology specimen. Y.L. assisted with biostatistical analysis. All authors reviewed the manuscript. |
ISSN: | 0028-0836 1476-4687 |
DOI: | 10.1038/s41586-019-1836-5 |