Microbial diversity in saliva of oral squamous cell carcinoma

• Published in: FEMS immunology and medical microbiology Vol. 61; no. 3; pp. 269 - 277
• Main Authors: Pushalkar, Smruti, Mane, Shrinivasrao P, Ji, Xiaojie, Li, Yihong, Evans, Clive, Crasta, Oswald R, Morse, Douglas, Meagher, Robert, Singh, Anup, Saxena, Deepak
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.04.2011; Blackwell; Oxford University Press
• Subjects: 454 pyrosequencing; Aged; Aged, 80 and over; Bacteria; Bacteria - classification; Bacteria - genetics; Biodiversity; Biological and medical sciences; Carcinoma, Squamous Cell - microbiology; Cluster Analysis; Colonization; denaturing gradient gel electrophoresis; DNA sequencing; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Epithelial cells; Firmicutes; Fundamental and applied biological sciences. Psychology; Genera; High-Throughput Nucleotide Sequencing; Humans; Inflammation; Male; Medical sciences; Metagenome; microbial diversity; Microbiology; Microorganisms; Middle Aged; Molecular Sequence Data; Mouth Neoplasms - microbiology; Mucosa; Oral cancer; Oral carcinoma; Oral cavity; Oral squamous cell carcinoma; Phylogeny; Polymerase chain reaction; Relative abundance; RNA, Ribosomal, 16S - genetics; rRNA; Saliva; Saliva - microbiology; Species diversity; Squamous cell carcinoma; Tumors; denaturing gradient gel electrophoresis; 454 pyrosequencing; oral squamous cell carcinoma; microbial diversity; Immunology; Stomatology; Denaturing gradient gel electrophoresis; Oral cavity disease; Malignant tumor; Oral squamous cell carcinoma; Saliva; Cancer
• ISSN: 0928-8244; 1574-695X; 1574-695X; 2049-632X
• DOI: 10.1111/j.1574-695X.2010.00773.x

In the oral cavity, chronic inflammation has been observed at various stages of oral squamous cell carcinomas (OSCC). Such inflammation could result from persistent mucosal or epithelial cell colonization by microorganisms. There is increasing evidence of the involvement of oral bacteria in inflammation, warranting further studies on the association of bacteria with the progression of OSCC. The objective of this study was to evaluate the diversity and relative abundance of bacteria in the saliva of subjects with OSCC. Using 454 parallel DNA sequencing, ∼58 000 PCR amplicons that span the V4-V5 hypervariable region of rRNAs from five subjects were sequenced. Members of eight phyla (divisions) of bacteria were detected. The majority of classified sequences belonged to the phyla Firmicutes (45%) and Bacteroidetes (25%). Further, 52 different genera containing approximately 860 (16.51%) known species were identified and 1077 (67%) sequences belonging to various uncultured bacteria or unclassified groups. The species diversity estimates obtained with abundance-based coverage estimators and Chao1 were greater than published analyses of other microbial profiles from the oral cavity. Fifteen unique phylotypes were present in all three OSCC subjects.