蜂蜜中17种水解氨基酸的测定

目的采用酸水解法和全自动氨基酸分析仪同时测定蜂蜜中17种氨基酸的含量。方法 8种不同蜜源的蜂蜜经酸水解后,采用全自动氨基酸分析仪进行定量检测;检测器为荧光检测器,脯氨酸在波长440 nm处测定,其余16种氨基酸在570 nm波长下测定。结果 17种氨基酸的线性回归系数均达到0.99以上;方法检出限为5-10 nmoL/L,样品平均回收率为92.1%-99.3%,相对标准偏差RSD小于8.5%。经检测实际样品发现,蜂蜜中不含胱氨酸,氨基酸总量在32.214-78.439 mg/100 g范围。结论该方法快速、准确,可适用于蜂蜜中水解氨基酸含量的测定。...

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Bibliographic Details
Published in食品安全质量检测学报 Vol. 7; no. 10; pp. 4033 - 4037
Main Author 牛之瑞 冯雷 鲁燕骅 谭建林 王吉祥 晏龙 杨凡 王亚琴 王世波 张晓红
Format Journal Article
LanguageChinese
Published 云南省产品质量监督检验研究院，国家热带农副产品监督检验中心，昆明 650223 2016
Subjects
氨基酸分析仪
水解氨基酸
蜂蜜
水解氨基酸
amino acid analyzer
蜂蜜
honey
hydrolytic amino acids
氨基酸分析仪
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Summary:目的采用酸水解法和全自动氨基酸分析仪同时测定蜂蜜中17种氨基酸的含量。方法 8种不同蜜源的蜂蜜经酸水解后,采用全自动氨基酸分析仪进行定量检测;检测器为荧光检测器,脯氨酸在波长440 nm处测定,其余16种氨基酸在570 nm波长下测定。结果 17种氨基酸的线性回归系数均达到0.99以上;方法检出限为5-10 nmoL/L,样品平均回收率为92.1%-99.3%,相对标准偏差RSD小于8.5%。经检测实际样品发现,蜂蜜中不含胱氨酸,氨基酸总量在32.214-78.439 mg/100 g范围。结论该方法快速、准确,可适用于蜂蜜中水解氨基酸含量的测定。
Bibliography:NIU Zhi-Rui, FENG Lei, LU Yan-Hua, TAN Jian-Lin, WANG Ji-Xiang, YAN Long, YANG Fan, WANG Ya-Qin, WANG Shi-Bo, ZHANG Xiao-Hong （Yunnan Institute of Product Quality Supervision ＆ Inspection, National Agricultural and Sideline Products Quality Supervision and Inspection Center, Kunming 650223, China）
Objective To establish a method for simultaneous determination of 17 kinds of hydrolytic amino acids by acid hydrolyzation and amino acid analyzer.Methods Eight different sources of honey samples were hydrolyzed with acid,and the quantitative detection was carried out with a full automatic amino acid analyzer.Proline was detected by fluorescence detector at the wavelength of 440 nm while the other 16 kinds of amino acid were detected at the wavelength of 570 nm.Results Linear regression coefficients of 17 kinds of hydrolytic amino acids were all above 0.99,the limits of detection were 5-10 nmoL/L,and the recovery rates of samples were 92.1%-99.3%with RSDs less than 8.5%.By detecting the actual samples,it was found th
ISSN:2095-0381