高效液相色谱法测定番茄中腐霉利和异菌脲的残留量

目的建立固相萃取-高效液相色谱法测定番茄中腐霉利和异菌脲的残留量。方法番茄样品用乙腈超声提取,加入氯化钠进行盐析脱水,提取液过Florisil柱净化,用正己烷/丙酮（90：10,V：V）淋洗液洗脱,洗脱液氮吹近干,甲醇定容后经ZORBAX Eclipse XDB-C18（250 mm×4.6 mm,5μm）色谱柱进行分离,以乙腈/水（60：40,V：V）为流动相,流速为1.0 m L/min,采用二极管阵列检测器检测,检测波长为208 nm。结果在0.055.0 mg/L浓度范围内,腐霉利和异菌脲的质量浓度与其峰面积呈良好的线性关系（r2〉0.999）,方法检出限均为0.025 mg/kg,在...

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Bibliographic Details
Published in食品安全质量检测学报 Vol. 8; no. 2; pp. 486 - 490
Main Author 徐秋生 贺慧琳 朱吕 吴爱红
Format Journal Article
LanguageChinese
Published 平湖市食品药品检测中心,平湖,314200 2017
Subjects
异菌脲
残留量
番茄
腐霉利
高效液相色谱法
high performance liquid chromatography
残留量
番茄
iprodione
高效液相色谱法
腐霉利
tomato
异菌脲
procymidone
residue
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ISSN2095-0381

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Summary:目的建立固相萃取-高效液相色谱法测定番茄中腐霉利和异菌脲的残留量。方法番茄样品用乙腈超声提取,加入氯化钠进行盐析脱水,提取液过Florisil柱净化,用正己烷/丙酮（90：10,V：V）淋洗液洗脱,洗脱液氮吹近干,甲醇定容后经ZORBAX Eclipse XDB-C18（250 mm×4.6 mm,5μm）色谱柱进行分离,以乙腈/水（60：40,V：V）为流动相,流速为1.0 m L/min,采用二极管阵列检测器检测,检测波长为208 nm。结果在0.055.0 mg/L浓度范围内,腐霉利和异菌脲的质量浓度与其峰面积呈良好的线性关系（r2〉0.999）,方法检出限均为0.025 mg/kg,在0.05、0.5和1.0 mg/kg 3个不同加标水平上,腐霉利和异菌脲的加标回收率为90.6%102.4%,精密度为3.72%6.55%。结论该方法操作简单、准确,可应用于番茄中腐霉利和异菌脲的检测。
Bibliography:Objective To establish a method for determination of procymidone and iprodione residues in tomato by solid phase extraction-high performance liquid chromatography（SPE-HPLC）. Methods After ultrasonic extraction with acetonitrile, the tomato extracts were added sodium chloride to salt out, purified by Florisil column, eluted by the purified solution with n-hexane/acetone（90：10, V：V）, blown by nitrogen evaporator until it was almost dry and diluted with methyl alcohol to a constant volume. Then it was separated by a ZORBAX Eclipse XDB-C18column（250 mm×4.6 mm, 5 μm） using acetonitrile/water（60：40, V：V） as mobile phase at the flow rate of 1.0 m L/min, and detected by diode array detector（DAD） at 208 nm. Results Procymidone and iprodione had good linear relationships between mass concentration and peak area（r2 〉0.999） in the range of 0.055.0 mg/L. The detection limits of two pesticides were both 0.025 mg/kg. The recoveries spiked at 3 levels of 0.05, 0.5 and 1.0 mg/kg were 90.6%102.4% with RSD of 3.72%6.55%. Conclu
ISSN:2095-0381