碳离子束辐照大豆当代诱变效应及褐皮突变体的初步研究

利用不同剂量碳离子束辐照大豆“合丰55”干种子，通过对表型、株高、分枝数及产量相关性状的考察以及DNA指纹图谱分析探索离子束辐照大豆的当代诱变效应。结果表明离子束诱变大豆M1代田问出现多种变异表型，辐照处理对M1代株高和分枝数有抑制作用，单株荚数、单株粒数、单株粒重及百粒重随着辐照剂量的增加呈现先降低后升高再降低的趋势，但这些变异与对照相比并未达到显著水平。大豆种子经辐照后M1代筛选到1株褐皮突变体，种皮颜色由黄色变为褐色，而且这种变异在M2代能够稳定遗传。运用随机扩增多态性（Random amplified polymorphic DNA，RAPD）技术对其DNA多态性进行研究发现，辐照后突...

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Published in辐射研究与辐射工艺学报 Vol. 32; no. 2; pp. 53 - 59
Main Author 余丽霞 李文建 杜艳 刘瑞媛 周利斌 骆善伟 陈刚 刘青芳
Format Journal Article
LanguageChinese
Published 中国科学院大学 100049 北京%中国科学院近代物理研究所 730000 兰州%宁夏农林科学院 750002 银川 2014
中国科学院近代物理研究所 730000 兰州
Subjects
大豆
碳离子束
褐皮突变体
诱变效应
褐皮突变体
大豆
Brown seed vessel mutant
碳离子束
mutagenic effects
Carbon ions
诱变效应
Soybean
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ISSN1000-3436

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Summary:利用不同剂量碳离子束辐照大豆“合丰55”干种子，通过对表型、株高、分枝数及产量相关性状的考察以及DNA指纹图谱分析探索离子束辐照大豆的当代诱变效应。结果表明离子束诱变大豆M1代田问出现多种变异表型，辐照处理对M1代株高和分枝数有抑制作用，单株荚数、单株粒数、单株粒重及百粒重随着辐照剂量的增加呈现先降低后升高再降低的趋势，但这些变异与对照相比并未达到显著水平。大豆种子经辐照后M1代筛选到1株褐皮突变体，种皮颜色由黄色变为褐色，而且这种变异在M2代能够稳定遗传。运用随机扩增多态性（Random amplified polymorphic DNA，RAPD）技术对其DNA多态性进行研究发现，辐照后突变体DNA与对照相比扩增条带或增加或减少，相似性系数为0．9513。综合结果表明，碳离子束辐照对大豆当代产生了一定的诱变效应，不仅造成了农艺性状的变异，而且使DNA水平也发生了明显的变化。
Bibliography:YU Lixia, LI Wenjian, DU Yan,LIU Ruiyuan,ZHOU Libin, LUO Shanwei, CHEN Gang, LIU Qingfang（1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China） 2 （University of Chinese Academy of Sciences, Beijing 100049, China） 3 （Ningxia Academy of Agro-Forestry Sciences, Yinchuan 750002, China）
31-1258/TL
Carbon ions, mutagenic effects, Soybean, Brown seed vessel mutant
Dry seeds of soybean ＂Hefeng 55＂ were irradiated by carbon ions at different doses. The characters including phenotype, plant height, branch number, yield trait and DNA fingerprint were investigated to explore the M1 mutagenic effects. The results showed that various phenotypes appeared after irradiation by carbon ions. The plant height and branch number of M1 generation were significantly inhabited by carbon ions. The pods, seeds and seed weight per plant as well as hundred-seed weight showed the same trend of decrease-increase-decrease along with the dose increment. However, these variations did not reach a significant level. Am
ISSN:1000-3436