The Nucleotide and Predicted Amino Acid Sequence of the Fusion Protein of Recent Isolates of Canine Distemper Virus in Japan

• Published in: Journal of Veterinary Medical Science Vol. 60; no. 3; pp. 381 - 385
• Main Authors: IWATSUKI, Kiyoko, MIYASHITA, Naoko, YOSHIDA, Emi, SHIN, Yeon-Sil, OHASHI, Kenjiro, KAI, Chieko, MIKAMI, Takeshi
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.03.1998; Japan Science and Technology Agency
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Canine distemper virus; CDV; Distemper Virus, Canine - classification; Distemper Virus, Canine - genetics; Distemper Virus, Canine - isolation & purification; DNA Primers; Dogs; field isolate; fusion protein; Molecular Sequence Data; Phylogeny; Sequence Alignment; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Viral Fusion Proteins - biosynthesis; Viral Fusion Proteins - chemistry; Viral Fusion Proteins - genetics
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.60.381

Analysis of the molecular properties of fusion (F) proteins of field isolates of canine distemper virus (CDV) by immunoprecipitation analysis revealed an identical molecular mass of F protein of 3 field isolates as well as the Onderstepoort laboratory strain. Sequencing showed that the F gene of a field isolate (the Yanaka strain) shared 90.1% and 95.7% identities with the Onderstepoort strain at nucleotide and amino acid levels, respectively. All of the 13 cysteine residues and 4 potential asparagine-linked glycosylation sites were completely conserved amongst these strains. These results indicate that the F proteins is much less heterogeneous than that observed in the hemagglutinin proteins of CDV.