Optical monitoring of glutamate release at multiple synapses in situ detects changes following LTP induction

• Published in: Molecular brain Vol. 13; no. 1; pp. 39 - 10
• Main Authors: Kopach, Olga, Zheng, Kaiyu, Rusakov, Dmitri A.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 13.03.2020; BioMed Central; BMC
• Subjects: Acute hippocampal slices; Amino acids; Animals; Axons - metabolism; Binding sites; Biological Transport; Dependovirus - metabolism; Female; Glutamate; Glutamate release; Glutamic Acid - metabolism; Hippocampus - metabolism; Hypotheses; Lasers; Long-Term Potentiation; LTP; Male; Memory; Mice, Inbred C57BL; Optical glutamate sensor; Optical Imaging; Optical scanners; Sensors; Synapses; Synapses - metabolism; Two-photon excitation imaging; Acute hippocampal slices; Optical glutamate sensor; LTP; Glutamate release; Two-photon excitation imaging
• ISSN: 1756-6606; 1756-6606
• DOI: 10.1186/s13041-020-00572-x

Information processing and memory formation in the brain relies on release of the main excitatory neurotransmitter glutamate from presynaptic axonal specialisations. The classical Hebbian paradigm of synaptic memory, long-term potentiation (LTP) of transmission, has been widely associated with an increase in the postsynaptic receptor current. Whether and to what degree LTP induction also enhances presynaptic glutamate release has been the subject of debate. Here, we took advantage of the recently developed genetically encoded optical sensors of glutamate (iGluSnFR) to monitor its release at CA3-CA1 synapses in acute hippocampal slices, before and after the induction of LTP. We attempted to trace release events at multiple synapses simultaneously, by using two-photon excitation imaging in fast frame-scanning mode. We thus detected a significant increase in the average iGluSnFR signal during potentiation, which lasted for up to 90 min. This increase may reflect an increased amount of released glutamate or, alternatively, reduced glutamate binding to high-affinity glutamate transporters that compete with iGluSnFR.