Ultraviolet radiation damages self noncoding RNA and is detected by TLR3

• Published in: Nature medicine Vol. 18; no. 8; pp. 1286 - 1290
• Main Authors: Bernard, Jamie J, Cowing-Zitron, Christopher, Nakatsuji, Teruaki, Muehleisen, Beda, Muto, Jun, Borkowski, Andrew W, Martinez, Laisel, Greidinger, Eric L, Yu, Benjamin D, Gallo, Richard L.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.08.2012; Nature Publishing Group
• Subjects: 631/250/262/2106/2108; 631/337/384; 692/420/256; Adaptor Proteins, Vesicular Transport - deficiency; Adaptor Proteins, Vesicular Transport - physiology; Aging; Animals; Biomedical and Life Sciences; Biomedicine; Cancer Research; Carcinogenesis; Cytokines; Exposure; Female; Gene Expression Regulation - radiation effects; Health aspects; Humans; Immune system; Infectious Diseases; Inflammation; Interleukin-6 - biosynthesis; Interleukin-6 - genetics; Irradiation; Keratinocytes - metabolism; Keratinocytes - radiation effects; letter; Leukocytes - metabolism; Leukocytes - radiation effects; Male; Metabolic Diseases; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular Medicine; Neurosciences; Ribonucleic acid; RNA; RNA, Double-Stranded - genetics; RNA, Double-Stranded - radiation effects; RNA, Small Interfering - pharmacology; RNA, Small Nuclear - genetics; RNA, Small Nuclear - radiation effects; Skin; Skin - radiation effects; Sunburn & sun tanning; Synthesis; Toll-Like Receptor 3 - antagonists & inhibitors; Toll-Like Receptor 3 - deficiency; Toll-Like Receptor 3 - genetics; Toll-Like Receptor 3 - physiology; Tumor necrosis factor; Tumor Necrosis Factor-alpha - biosynthesis; Tumor Necrosis Factor-alpha - genetics; Ultraviolet radiation; Ultraviolet Rays - adverse effects; Up-Regulation - radiation effects; United States
• ISSN: 1078-8956; 1546-170X
• DOI: 10.1038/nm.2861

Ultraviolet radiation induces an inflammatory response in the skin, but it remains unclear how cells in the skin detect this damage and trigger an inflammatory response. Richard L. Gallo and his colleagues report that ultraviolet radiation damages self noncoding RNA. These modified RNA are released from irradiated keratinocytes and act as a danger signal that is detected by Toll-like receptor 3 (TLR3), which is required for the induction of proinflammatory cytokine release and for radiation-induced immune suppression. Exposure to ultraviolet B (UVB) radiation from the sun can result in sunburn, premature aging and carcinogenesis, but the mechanism responsible for acute inflammation of the skin is not well understood. Here we show that RNA is released from keratinocytes after UVB exposure and that this stimulates production of the inflammatory cytokines tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) from nonirradiated keratinocytes and peripheral blood mononuclear cells (PBMCs). Whole-transcriptome sequencing revealed that UVB irradiation of keratinocytes induced alterations in the double-stranded domains of some noncoding RNAs. We found that this UVB-damaged RNA was sufficient to induce cytokine production from nonirradiated cells, as UVB irradiation of a purified noncoding RNA (U1 RNA) reproduced the same response as the one we observed to UVB-damaged keratinocytes. The responses to both UVB-damaged self-RNAs and UVB-damaged keratinocytes were dependent on Toll-like receptor 3 (TLR3) and Toll-like receptor adaptor molecule 1 (TRIF). In response to UVB exposure, Tlr3 −/− mice did not upregulate TNF-α in the skin. Moreover, TLR3 was also necessary for UVB-radiation–induced immune suppression. These findings establish that UVB damage is detected by TLR3 and that self-RNA is a damage-associated molecular pattern that serves as an endogenous signal of solar injury.