Anatabine ameliorates intestinal inflammation and reduces the production of pro-inflammatory factors in a dextran sulfate sodium mouse model of colitis

• Published in: Journal of inflammation (London, England) Vol. 17; no. 1; p. 29
• Main Authors: Ruiz Castro, Pedro A, Kogel, Ulrike, Lo Sasso, Giuseppe, Phillips, Blaine W, Sewer, Alain, Titz, Bjorn, Garcia, Llenalia, Kondylis, Athanasios, Guedj, Emmanuel, Peric, Dariusz, Bornand, David, Dulize, Remi, Merg, Celine, Corciulo, Maica, Ivanov, Nikolai V, Peitsch, Manuel C, Hoeng, Julia
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 24.08.2020; BioMed Central; BMC
• Subjects: Abundance; Alkaloids; Analysis; Animal models; Anti-inflammatory agents; Cell adhesion & migration; Colitis; Colon; Crohn's disease; Dextran; Dextran sulfate; Dextrans; Disease; Drinking water; Environmental factors; Etiology; Gene expression; Inflammation; Inflammatory bowel disease; Inflammatory bowel diseases; Interleukin 10; Internet resources; Intestine; Medical research; Mouse model; Nicotine; Oral administration; Plant-derived alkaloid; Principal components analysis; Production management; Sulfates; Tobacco; Ulcerative colitis; Youth smoking; Mouse model; Colitis; Nicotine; Plant-derived alkaloid
• ISSN: 1476-9255; 1476-9255
• DOI: 10.1186/s12950-020-00260-6

Inflammatory bowel disease (IBD) is the collective term for chronic immune-mediated diseases of unknown, multifactorial etiology, arising from the interplay between genetic and environmental factors and including two main disease manifestations: ulcerative colitis (UC) and Crohn's disease. In the last few decades, naturally occurring alkaloids have gained interest because of their substantial anti-inflammatory effects in several animal models of disease. Studies on mouse models of IBD have demonstrated the anti-inflammatory action of the main tobacco alkaloid, nicotine. In addition, anatabine, a minor tobacco alkaloid also present in peppers, tomato, and eggplant presents anti-inflammatory properties in vivo and in vitro. In this study, we aimed to evaluate the anti-inflammatory properties of nicotine and anatabine in a dextran sulfate sodium (DSS) mouse model of UC. Oral administration of anatabine, but not nicotine, reduced the clinical symptoms of DSS-induced colitis. The result of gene expression analysis suggested that anatabine had a restorative effect on global DSS-induced gene expression profiles, while nicotine only had limited effects. Accordingly, MAP findings revealed that anatabine reduced the colonic abundance of DSS-associated cytokines and increased IL-10 abundance. Our results support the amelioration of inflammatory effects by anatabine in the DSS mouse model of UC, and suggest that anatabine constitutes a promising therapeutic agent for IBD treatment.