TU-tagging: cell type-specific RNA isolation from intact complex tissues

We found that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil delivery can be used to label and purify cell type-specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell types t...

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Published inNature methods Vol. 6; no. 6; pp. 439 - 441
Main Authors Doe, Chris Q, Miller, Michael R, Robinson, Kristin J, Cleary, Michael D
Format Journal Article
LanguageEnglish
Published New York Nature Publishing Group US 01.06.2009
Nature Publishing Group
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Summary:We found that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil delivery can be used to label and purify cell type-specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in many organisms, including mammals and other vertebrates.
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Current address: School of Natural Sciences, University of California, Merced, CA 95344, USA
ISSN:1548-7091
1548-7105
DOI:10.1038/nmeth.1329