Generation of Monoclonal Antibodies against a Feline CD Antigen (CD4) Expressed by a Recombinant Baculovirus

We attempted to establish a system to generate monoclonal antibodies (mAbs) recognizing CD antigens on lymphocytes of domestic animals using the expressed CD antigens by a baculovirus expression system. For this purpose, we selected feline CD4 (fCD4) antigen and expressed it in an insect cell line (...

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Published inJournal of Veterinary Medical Science Vol. 59; no. 6; pp. 467 - 469
Main Authors SHIMOJIMA, Masayuki, MORIKAWA, Shigeru, MAEDA, Ken, TOHYA, Yukinobu, MIYAZAWA, Takayuki, MIKAMI, Takeshi
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.06.1997
Japan Science and Technology Agency
Subjects
Animals
Antibodies, Monoclonal - biosynthesis
Baculoviridae - immunology
Baculovirus
Cats
CD4 Antigens - immunology
Cell Line
feline CD4 antigen
Insecta
Lymphocytes - immunology
Mice
Mice, Inbred BALB C
monoclonal antibody
Recombinant Proteins - biosynthesis
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Summary:We attempted to establish a system to generate monoclonal antibodies (mAbs) recognizing CD antigens on lymphocytes of domestic animals using the expressed CD antigens by a baculovirus expression system. For this purpose, we selected feline CD4 (fCD4) antigen and expressed it in an insect cell line (Sf9 cells). To obtain mAbs, BALB/c mice were immunized with feline peripheral blood mononuclear cells (fPBMCs) or Sf9 cells expressing the fCD4 (Sf-fCD4 cells), and then hybridomas secreting antibodies were screened by indirect immunofluorescence assay against the opposite antigens of Sf-fCD4 cells or fPBMCs, respectively. Five mAbs recognizing the fCD4 were obtained in total. The system established here might be useful to obtain mAbs recognizing CD antigens of domestic animals.
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ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.59.467