Production of Monoclonal Antibody Discriminating Serological Difference in Escherichia coli O9 and O9a Polysaccharides

A monoclonal antibody (mAb) with a unique antigenic specificity against Escherichia coli O9 was produced. The O9a mAb was reactive with a part of the strains in E. coli O9. The O9a mAb did not react with LPS from the E. coli O9 test strain Bi316-42. The distribution of the antigen defined by the O9a...

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Published inMICROBIOLOGY and IMMUNOLOGY Vol. 41; no. 7; pp. 519 - 525
Main Authors Kido, Nobuo, Morooka, Naoki, Paeng, Noriko, Ohtani, Taizo, Kobayashi, Hidemitsu, Shibata, Nobuyuki, Okawa, Yoshio, Suzuki, Shigeo, Sugiyama, Tsuyoshi, Yokochi, Takashi
Format Journal Article
LanguageEnglish
Published Tokyo Blackwell Publishing Ltd 01.01.1997
Center For Academic Publications Japan
Center for Academic Publications Japan
Subjects
Animals
Antibodies, Monoclonal - biosynthesis
Antibody Specificity
Bacteriological methods and techniques used in bacteriology
Bacteriology
Binding Sites, Antibody
Biological and medical sciences
Epitopes - analysis
Epitopes - chemistry
Escherichia coli
Escherichia coli - classification
Escherichia coli - immunology
Fundamental and applied biological sciences. Psychology
Immunoblotting
Lipopolysaccharide
Magnetic Resonance Spectroscopy
Mice
Mice, Inbred BALB C
Microbiology
O antigen
O Antigens - analysis
O polysaccharide
Rabbits
Serotype
O antigen
Serotype specificity
Escherichia coli
Bacteria
Monoclonal antibody
Polysaccharide
Enterobacteriaceae
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Summary:A monoclonal antibody (mAb) with a unique antigenic specificity against Escherichia coli O9 was produced. The O9a mAb was reactive with a part of the strains in E. coli O9. The O9a mAb did not react with LPS from the E. coli O9 test strain Bi316-42. The distribution of the antigen defined by the O9a mAb in E. coli O9 was consistent with that of E. coli O9a present in E. coli O9 strains. The chemical structure of the repeating unit of the O-specific polysaccharide detected by the mAb was demonstrated to be a mannotetraose by two-dimensional nuclear magnetic resonance spectroscopy. It was confirmed that the mAb recognized E. coli O9a serotype in E. coli O9 serotype strains, suggesting that E. coli O9a serotype might be a dominant strain in E. coli O9.
Bibliography:ark:/67375/WNG-9TWX4R9Q-C
ArticleID:MIM01887
istex:02A96B24858FCE2FC9735720BBF4259E82CE3AB8
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
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ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1997.tb01887.x