Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans

• Published in: Acta crystallographica. Section F, Structural biology and crystallization communications Vol. 67; no. 12; pp. 1542 - 1544
• Main Authors: Suzuki, Nobuhiro, Kim, Young-Min, Fujimoto, Zui, Momma, Mitsuru, Kang, Hee-Kwon, Funane, Kazumi, Okuyama, Masayuki, Mori, Haruhide, Kimura, Atsuo
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.12.2011
• Subjects: Crystallization; Crystallization Communications; Crystallography, X-Ray; dextran; Dextranase - chemistry; dextranases; glycoside hydrolase family 66; isomaltooligosaccharides; Streptococcus mutans; Streptococcus mutans - enzymology
• ISSN: 1744-3091; 1744-3091
• DOI: 10.1107/S1744309111038425

Streptococcus mutans dextranase hydrolyzes the internal α‐1,6‐linkages of dextran and belongs to glycoside hydrolase family 66. An N‐ and C‐terminal deletion mutant of S. mutans dextranase was crystallized by the sitting‐drop vapour‐diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P21, with unit‐cell parameters a = 53.2, b = 89.7, c = 63.3 Å, β = 102.3°. Assuming that the asymmetric unit of the crystal contained one molecule, the Matthews coefficient was calculated to be 4.07 Å3 Da−1; assuming the presence of two molecules in the asymmetric unit it was calculated to be 2.03 Å3 Da−1.