Perilymphatic potassium changes and potassium homeostasis in isolated semicircular canals of the frog
1. Endolymphatic and perilymphatic potassium concentrations were measured with K(+)-sensitive microelectrodes in isolated semicircular canals of the frog. K+ levels were evaluated both at rest and during sinusoidal stimulation (0.05 Hz) of the sensory organ. 2. Mechanical stimulation of hair cells w...
Saved in:
Published in | The Journal of physiology Vol. 430; no. 1; pp. 585 - 594 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Oxford
The Physiological Society
01.11.1990
Blackwell |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | 1. Endolymphatic and perilymphatic potassium concentrations were measured with K(+)-sensitive microelectrodes in isolated
semicircular canals of the frog. K+ levels were evaluated both at rest and during sinusoidal stimulation (0.05 Hz) of the
sensory organ. 2. Mechanical stimulation of hair cells was associated with sinusoidal changes (about 0.2 mM) in the perilymphatic
K+ concentration. 3. Perilymphatic K(+)-fluctuations were modified neither by impairment of the synaptic transmission at cyto-neural
junctions nor by chronic denervation of the crista ampullaris, thus indicating that K+ ions were actually released by hair
cells. 4. Voltage-clamp experiments of the whole sensory organ showed that K+ flows across the crista ampullaris can vary
from 3 X 10(11) molecules of K+ s-1 at rest up to about 15 X 10(11) molecules of K+ s-1 during mechanical stimuli. 5. Measurement
of intra-ampullar K+ concentration demonstrated that the amount of K+ transported from the perilymph towards the endolymph
can be rapidly altered by modifying its perilymphatic levels. This suggests that vestibular organs are endowed with K+ homeostatic
mechanisms able to buffer in a very efficient way the concentration of K+ in both the fluids bathing the crista ampullaris.
6. The possible role of K+ homeostatic mechanisms in hair cell adaptation is discussed. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-3751 1469-7793 |
DOI: | 10.1113/jphysiol.1990.sp018308 |