小檗碱体外抗子宫内膜癌HEC-1A细胞的作用研究

目的研究小檗碱体外对非激素依赖型人子宫内膜癌HEC-1A细胞增殖、迁移、周期、凋亡的影响。方法 MTT法检测小檗碱对人子宫内膜癌HEC-1A[中分化腺癌,雌激素受体（ER）弱阳性,孕激素受体（PR）阴性]细胞株的抑制作用;倒置显微镜观察细胞形态变化;将实验组分成50、25、12.5 mg/L高、中、低剂量组。Transwell法测定细胞体外迁移能力,流式细胞仪观察细胞周期分布,Annexin V-FITC/PI双染检测HEC-1A细胞凋亡。结果小檗碱可显著抑制HEC-1A细胞生长,在100、50、25、12.5、6.25 mg/L浓度范围内呈剂量依赖性（P〈0.05）。小檗碱能明显改变细胞形态...

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Published in北京中医药大学学报 Vol. 40; no. 12; pp. 1018 - 1024
Main Author 鲁周南;包晓霞;薛晓鸥;唐瑶
Format Journal Article
LanguageChinese
Published 北京中医药大学 北京100029%北京中医药大学东直门医院 2017
Subjects
HEC-1A细胞株
凋亡
周期
子宫内膜癌
小檗碱
迁移
hormone-independent endometrial carcinoma
cell liner HEC-1 A
子宫内膜癌
cell cycle
cell migration
cell apoptosis
迁移
HEC-1A细胞株
凋亡
周期
小檗碱
berberine
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Summary:目的研究小檗碱体外对非激素依赖型人子宫内膜癌HEC-1A细胞增殖、迁移、周期、凋亡的影响。方法 MTT法检测小檗碱对人子宫内膜癌HEC-1A[中分化腺癌,雌激素受体（ER）弱阳性,孕激素受体（PR）阴性]细胞株的抑制作用;倒置显微镜观察细胞形态变化;将实验组分成50、25、12.5 mg/L高、中、低剂量组。Transwell法测定细胞体外迁移能力,流式细胞仪观察细胞周期分布,Annexin V-FITC/PI双染检测HEC-1A细胞凋亡。结果小檗碱可显著抑制HEC-1A细胞生长,在100、50、25、12.5、6.25 mg/L浓度范围内呈剂量依赖性（P〈0.05）。小檗碱能明显改变细胞形态,小檗碱作用于子宫内膜癌HEC-1A细胞后,倒置显微镜下可见部分细胞体积改变,细胞内出现空泡,结构破坏,裂解成碎片,贴壁细胞减少,并散在排列。抑制子宫内膜癌HEC-1A细胞体外迁移的能力,高剂量组效果更佳,迁移细胞数明显减少。小檗碱低剂量组可以将HEC-1A细胞阻滞于G0/G1期。小檗碱作用24 h能提高HEC-1A细胞总凋亡率、早期凋亡率及晚期凋亡率,早期凋亡率更明显。结论小檗碱能够抑制非激素依赖型子宫内膜癌HEC-1A细胞株的增殖、迁移,并且促进HEC-1A细胞凋亡,低剂量小檗碱能将细胞阻滞在G0/G1期。
Bibliography:11-3574/R
berberine ; hormone-independent endometrial carcinoma ; cell liner HEC-1 A ; cell cycle ; cellmigration ; cell apoptosis
Objective To study the effects of berberine on proliferation,migration,cell cycle and apoptosis in hormone-independent endometrial carcinoma cell line HEC-1 A. Methods Berberine＇s inhibition of HEC-1 A cells proliferation was measured by using MTT colorimetry assay. Cell morphology was inspected under an inverted microscope. Investigated Berberine groups were divided into high-dose group（ 50 mg/L）,mid-dose group（ 25 mg/L）,and low-dose group（ 12. 5 mg/L）. Migration ability of cells in vitro was determined with Transwell. Cell cycle expressions were observed by using flow cytometry.Apoptosis of HEC-1 A cells was detected with Annexin V-FITC/PI double staining. Results Berberine may significantly inhibit the proliferation of HEC-1 A cells in a dose-dependent manner（ effective concentration of berberine ranged between 100 mg/L to 6. 25 mg/L）（ P 0. 05）. Berberine could markedly change ce
ISSN:1006-2157
DOI:10.3969/j.issn.1006-2157.2017.12.009