CRISPR-induced exon skipping is dependent on premature termination codon mutations

In previous studies, CRISPR/Cas9 was shown to induce unexpected exon skipping; however, the mechanism by which this phenomenon is triggered is controversial. By analyzing 22 gene-edited rabbit lines generated using CRISPR/Cas9, we provide evidence of exon skipping at high frequency in premature term...

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Published inGenome Biology Vol. 19; no. 1; p. 164
Main Authors Sui, Tingting, Song, Yuning, Liu, Zhiquan, Chen, Mao, Deng, Jichao, Xu, Yuanyuan, Lai, Liangxue, Li, Zhanjun
Format Journal Article
LanguageEnglish
Published England BioMed Central 17.10.2018
BMC
Subjects
Animals
Clustered Regularly Interspaced Short Palindromic Repeats - genetics
Codon, Nonsense - genetics
CRISPR
CRISPR/Cas9
Exon skipping
Exons - genetics
Frameshift mutation
Genes
Genomes
Missense mutation
Mutation
Nonsense mutation
PTC
Rabbits
Short Report
Splicing
Rabbits
CRISPR/Cas9
Exon skipping
PTC
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Summary:In previous studies, CRISPR/Cas9 was shown to induce unexpected exon skipping; however, the mechanism by which this phenomenon is triggered is controversial. By analyzing 22 gene-edited rabbit lines generated using CRISPR/Cas9, we provide evidence of exon skipping at high frequency in premature termination codon-mutated rabbits but not in the rabbits with a premature termination codon mutation in exon 1 rabbits with non-frameshift or missense mutations. Our results suggest that CRISPR-mediated exon skipping depends on premature termination codon mutation-induced nonsense-associated altered splicing.
Bibliography:ObjectType-Article-1
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content type line 23
ISSN:1474-760X
1474-7596
1474-760X
DOI:10.1186/s13059-018-1532-z