Decreasing miRNA sequencing bias using a single adapter and circularization approach

The ability to accurately quantify all the microRNAs (miRNAs) in a sample is important for understanding miRNA biology and for development of new biomarkers and therapeutic targets. We develop a new method for preparing miRNA sequencing libraries, RealSeq®-AC, that involves ligating the miRNAs with...

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Bibliographic Details
Published inGenome Biology Vol. 19; no. 1; p. 105
Main Authors Barberán-Soler, Sergio, Vo, Jenny M, Hogans, Ryan E, Dallas, Anne, Johnston, Brian H, Kazakov, Sergei A
Format Journal Article
LanguageEnglish
Published England BioMed Central 03.09.2018
BMC
Subjects
Bias
Biomarkers
Brain Chemistry
Efficiency
Genomes
Humans
Medical research
Method
Methods
MicroRNAs
MicroRNAs - analysis
MicroRNAs - chemistry
miRNA
miRNA-seq
Protocol
Sequence Analysis, RNA - methods
Sequencing bias
Small RNA library preparation
Small RNA sequencing
Therapeutic applications
Small RNA library preparation
miRNA-seq
Sequencing bias
Small RNA sequencing
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Summary:The ability to accurately quantify all the microRNAs (miRNAs) in a sample is important for understanding miRNA biology and for development of new biomarkers and therapeutic targets. We develop a new method for preparing miRNA sequencing libraries, RealSeq®-AC, that involves ligating the miRNAs with a single adapter and circularizing the ligation products. When compared to other methods, RealSeq®-AC provides greatly reduced miRNA sequencing bias and allows the identification of the largest variety of miRNAs in biological samples. This reduced bias also allows robust quantification of miRNAs present in samples across a wide range of RNA input levels.
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ISSN:1474-760X
1474-7596
1474-760X
DOI:10.1186/s13059-018-1488-z