PASTMUS: mapping functional elements at single amino acid resolution in human cells

• Published in: Genome Biology Vol. 20; no. 1; pp. 279 - 16
• Main Authors: Zhang, Xinyi, Yue, Di, Wang, Yinan, Zhou, Yuexin, Liu, Ying, Qiu, Yeting, Tian, Feng, Yu, Ying, Zhou, Zhuo, Wei, Wensheng
• Format: Journal Article
• Language: English
• Published: England BioMed Central 16.12.2019; BMC
• Subjects: Amino Acid Sequence; Amino acids; Bioinformatics; Cell Cycle Proteins - chemistry; CRISPR; Deoxyribonucleic acid; DNA; Genes; Genomes; Genotype & phenotype; Heparin-binding EGF-like Growth Factor - chemistry; Humans; Method; Mutagenesis; Mutation; Nucleotide sequence; Polo-Like Kinase 1; Protein Serine-Threonine Kinases - chemistry; Proteins; Proto-Oncogene Proteins - chemistry; Sequence Analysis, Protein - methods; Structure-Activity Relationship
• ISSN: 1474-760X; 1474-7596; 1474-760X
• DOI: 10.1186/s13059-019-1897-7

Identification of functional elements for a protein of interest is important for achieving a mechanistic understanding. However, it remains cumbersome to assess each and every amino acid of a given protein in relevance to its functional significance. Here, we report a strategy, PArsing fragmented DNA Sequences from CRISPR Tiling MUtagenesis Screening (PASTMUS), which provides a streamlined workflow and a bioinformatics pipeline to identify critical amino acids of proteins in their native biological contexts. Using this approach, we map six proteins-three bacterial toxin receptors and three cancer drug targets, and acquire their corresponding functional maps at amino acid resolution.