MiR-301b promotes the proliferation, mobility, and epithelial-to-mesenchymal transition of bladder cancer cells by targeting EGR1

• Published in: Biochemistry and cell biology Vol. 95; no. 5; pp. 571 - 577
• Main Authors: Yan, Lei, Wang, Yan, Liang, Jun, Liu, Zhixin, Sun, Xiaodong, Cai, Kerui
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.10.2017; Canadian Science Publishing NRC Research Press
• Subjects: Apoptosis; Assaying; Bladder; Bladder cancer; Cancer; Cancer cells; cancer de la vessie; Cell cycle; Cell Line, Tumor; Cell Movement; Cell Proliferation; Cytometry; Early Growth Response Protein 1 - biosynthesis; Early Growth Response Protein 1 - genetics; EGR-1 protein; EGR1; Epithelial-Mesenchymal Transition; epithelial-to-mesenchymal transition; Flow cytometry; Gene expression; Genetic aspects; Health aspects; Humans; Invasiveness; Mesenchyme; Methods; MicroRNA; MicroRNAs - genetics; MicroRNAs - metabolism; miR-301b; mobility; mobilité; mRNA; Polymerase chain reaction; Proteins; Reporter gene; Ribonucleic acid; RNA; RNA sequencing; Signaling; Target recognition; transition épithélio-mésenchymateuse; Urinary bladder; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - pathology; Wound healing; EGR1; mobility; mobilité; bladder cancer; miR-301b; cancer de la vessie; epithelial-to-mesenchymal transition; transition épithélio-mésenchymateuse
• ISSN: 0829-8211; 1208-6002
• DOI: 10.1139/bcb-2016-0232

We investigated the role of miR-301b in the modulation of the proliferation, migration, and invasion of bladder cancer (BLCA) cells. The expression of miR-301b and EGR1 (early growth response gene 1) mRNA were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). A dual-luciferase reporter gene system was used to identify the target relationship between miR-301b and EGR1. Cell proliferation, cell cycle, and apoptosis were analyzed by MTT assay, colony-forming assay, and flow cytometry, respectively. Cell motility and invasiveness were assessed by wound healing and Transwell assays. The expression of proteins involved in epithelial-to-mesenchymal transition (EMT) and EGR1 were determined by Western blot. Our results showed that miR-301b was up-regulated while EGR1 was down-regulated in BLCA tissues compared with adjacent normal tissues. The proliferation, migration, and invasiveness of T24 cells (a kind of human BLCA cell) were suppressed by decreasing miR-301b expression or increasing EGR1 expression. In addition, miR-301b promoted EMT signaling by influencing the expression of related proteins. In conclusion, miR-301b promotes the proliferation, migration, and aggressiveness of human BLCA cells by inhibiting the expression of EGR1.