Akt phosphorylates acinus and inhibits its proteolytic cleavage, preventing chromatin condensation

• Published in: The EMBO journal Vol. 24; no. 20; pp. 3543 - 3554
• Main Authors: Hu, Yuanxin, Yao, Joyce, Liu, Zhixue, Liu, Xia, Fu, Haian, Ye, Keqiang
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 19.10.2005; Blackwell Publishing Ltd
• Subjects: acinus; Akt; Animals; Cell Nucleus - chemistry; Cell Nucleus - metabolism; Chromatin - metabolism; chromatin condensation; DNA Fragmentation; Humans; Mutation; Nuclear Proteins - analysis; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Phosphorylation; Proto-Oncogene Proteins c-akt - metabolism; Rats; RNA Interference; Survival
• ISSN: 0261-4189; 1460-2075
• DOI: 10.1038/sj.emboj.7600823

Akt promotes cell survival by phosphorylating and inhibiting components of the intrinsic cell death machinery. Akt translocates into the nucleus upon exposure of cells to survival factors, but little is known about its functions in the nucleus. Here, we show that acinus, a nuclear factor required for apoptotic chromatin condensation, is a direct target of Akt. We demonstrate that Akt phosphorylation of acinus on serine 422 and 573 results in its resistance to caspase cleavage in the nucleus and the inhibition of acinus‐dependent chromatin condensation. Abolishing acinus phosphorylation by Akt through mutagenesis accelerates its proteolytic degradation and chromatin condensation. Acinus S422, 573D, a mutant mimicking phosphorylation, resists against apoptotic cleavage and prevents chromatin condensation. Knocking down of acinus substantially decreases chromatin condensation, and depletion of Akt provokes the apoptotic cleavage of acinus. Thus, Akt inhibits chromatin condensation during apoptosis by phosphorylating acinus in the nucleus, revealing a specific mechanism by which nuclear Akt promotes cell survival.