INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana

• Published in: PLoS pathogens Vol. 15; no. 1; p. e1007499
• Main Authors: Völz, Ronny, Kim, Soon-Kap, Mi, Jianing, Rawat, Anamika A, Veluchamy, Alaguraj, Mariappan, Kiruthiga G, Rayapuram, Naganand, Daviere, Jean-Michel, Achard, Patrick, Blilou, Ikram, Al-Babili, Salim, Benhamed, Moussa, Hirt, Heribert
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.01.2019; Public Library of Science (PLoS)
• Subjects: Analysis; Arabidopsis; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - immunology; Arabidopsis Proteins - genetics; Arabidopsis thaliana; Binding; Binding sites; Biochemistry, Molecular Biology; Bioinformatics; Biological activity; Biology and Life Sciences; Birds; Botanics; Deoxyribonucleic acid; Disease Resistance - immunology; DNA; DNA binding proteins; Engineering; Environmental science; Gene expression; Gene Expression Regulation, Plant - genetics; Gene mutation; Genetic aspects; Genomes; Genomics; Growth; Id1 protein; Immune response; Immune system; Immunity; Kinases; Life Sciences; MAP kinase; Medicine and Health Sciences; Microbial drug resistance; Mimicry; Molecular biology; Mutants; Mutation; Phenols (Class of compounds); Phosphorylation; Physiological aspects; Plant Development - genetics; Plant Diseases - genetics; Plant Immunity - genetics; Plant-pathogen relationships; Plants, Genetically Modified - metabolism; Proteins; Pseudomonas syringae; Research and Analysis Methods; Salicylic acid; Software; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Vegetal Biology; Saudi Arabia; France; transcription factor; functional analyses; receptor-like kinases; innate immunity; activated protein-kinases; quantitative phosphoproteomics; salicylic-acglucosyltransferase; gene-expression; dna-binding; phosphoproteome profiling reveals
• ISSN: 1553-7374; 1553-7366; 1553-7374
• DOI: 10.1371/journal.ppat.1007499

INDETERMINATE DOMAIN (IDD)/ BIRD proteins are a highly conserved plant-specific family of transcription factors which play multiple roles in plant development and physiology. Here, we show that mutation in IDD4/IMPERIAL EAGLE increases resistance to the hemi-biotrophic pathogen Pseudomonas syringae, indicating that IDD4 may act as a repressor of basal immune response and PAMP-triggered immunity. Furthermore, the idd4 mutant exhibits enhanced plant-growth indicating IDD4 as suppressor of growth and development. Transcriptome comparison of idd4 mutants and IDD4ox lines aligned to genome-wide IDD4 DNA-binding studies revealed major target genes related to defense and developmental-biological processes. IDD4 is a phospho-protein that interacts and becomes phosphorylated on two conserved sites by the MAP kinase MPK6. DNA-binding studies of IDD4 after flg22 treatment and with IDD4 phosphosite mutants show enhanced binding affinity to ID1 motif-containing promoters and its function as a transcriptional regulator. In contrast to the IDD4-phospho-dead mutant, the IDD4 phospho-mimicking mutant shows altered susceptibility to PstDC3000, salicylic acid levels and transcriptome reprogramming. In summary, we found that IDD4 regulates various hormonal pathways thereby coordinating growth and development with basal immunity.