In vitro thermotherapy-based methods for plant virus eradication

• Published in: Plant methods Vol. 14; no. 1; p. 87
• Main Authors: Wang, Min-Rui, Cui, Zhen-Hua, Li, Jing-Wei, Hao, Xin-Yi, Zhao, Lei, Wang, Qiao-Chun
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 06.10.2018; BioMed Central; BMC
• Subjects: Agricultural production; Botanical research; breeding; Chemotherapy; Control; Crop diseases; Crops; cryosurgery; Cryotherapy; Cultivars; Disease control; drug therapy; Economic importance; Flowers & plants; Fruits; Germplasm; Heat; imports; In vitro methods and tests; Infections; Methods; Micrografting; MicroRNAs; plant germplasm; Plant viruses; Review; RNA polymerase; Shoot tip culture; shoot tips; Sugarcane; Thermotherapy; Tobacco; Viral diseases; Virus diseases of plants; Virus eradication; virus-free plants; Viruses; United States > US; Europe; Chemotherapy; Micrografting; Thermotherapy; Cryotherapy; Shoot tip culture; Virus eradication
• ISSN: 1746-4811; 1746-4811
• DOI: 10.1186/s13007-018-0355-y

Production of virus-free plants is necessary to control viral diseases, import novel cultivars from other countries, exchange breeding materials between countries or regions and preserve plant germplasm. In vitro techniques represent the most successful approaches for virus eradication. In vitro thermotherapy-based methods, including combining thermotherapy with shoot tip culture, chemotherapy, micrografting or shoot tip cryotherapy, have been successfully established for efficient eradication of various viruses from almost all of the most economically important crops. The present study reviewed recent advances in in vitro thermotherapy-based methods for virus eradication since the twenty-first century. Mechanisms as to why thermotherapy-based methods could efficiently eradicate viruses were discussed. Finally, future prospects were proposed to direct further studies.