Identification of a novel canine parvovirus type 2c in Taiwan

• Published in: Virology journal Vol. 13; no. 1; p. 160
• Main Authors: Chiang, Shu-Yun, Wu, Hung-Yi, Chiou, Ming-Tang, Chang, Min-Chen, Lin, Chao-Nan
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 23.09.2016; BioMed Central
• Subjects: Carnivore protoparvovirus 1; Deoxyribonucleic acid; Diarrhea; DNA; Dogs; Evolution & development; Gene amplification; Genetic aspects; Genetic variation; Genomes; Infections; monitoring; Parvoviruses; Phylogenetics; phylogeny; Physiological aspects; puppies; quantitative polymerase chain reaction; sequence analysis; Surveillance; Taiwan; Taiwan; Brazil; Italy; Argentina; Uruguay; China; Germany; Canine parvovirus; CPV-2c; Taiwan
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/s12985-016-0620-5

Taiwan has been considered free from canine parvovirus type 2c (CPV-2c) based on the last report of canine parvovirus type 2 (CPV-2) surveillance. However, since January 2015, the first report of CPV-2c in a puppy has occurred in Taiwan. There is currently limited information about the CPV-2c variant in Taiwan. In the present study, we characterized the previously unidentified CPV-2c variant and investigated the distribution of CPV-2 variants in Taiwan. During January 2014 to April 2016, fecal or rectal swab samples from 99 dogs with suspected CPV-2 infection in Taiwan were collected. Eighty-eight were identified as being either CPV-2a, -2b or -2c variants positive by real-time PCR and sequence analysis. Sequence analysis of the 88 isolates confirmed CPV-2c as the dominant variant (54.6 %), followed by CPV-2b (26.1 %) and CPV-2a (19.3 %). Phylogenetic analysis demonstrated that the recent CPV-2c variants are similar to the Chinese CPV-2c strain but can be considered as novel Asian CPV-2c isolates. The present study provides evidence for the existence of a novel CPV-2c variant in Taiwan.