Plesiomonas shigelloides exports a lethal cytotoxic-enterotoxin (LCE) by membrane vesicles

Plesiomonas shigelloides isolated from water in Brazil was previously described as a hemorrhagic heat-labile cytotoxic-enterotoxin producer. We purified this toxin from culture supernatants using ion metallic affinity chromatography (IMAC) followed by molecular exclusion chromatography. The pure tox...

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Published inThe Brazilian journal of infectious diseases Vol. 20; no. 6; pp. 546 - 555
Main Authors Ludovico, Marilucia Santos, Martins, Luciano Moura, Bianco, Juares Ednaldo Romero, Andrade, Célia Guadalupe Tardelli de Jesus, Falcon, Rosabel, Joazeiro, Paulo Pinto, Gatti, Maria Silvia Viccari, Yano, Tomomasa
Format Journal Article
LanguageEnglish
Portuguese
Published Brazil Elsevier Editora Ltda 01.11.2016
Contexto
Elsevier
Brazilian Society of Infectious Diseases
Subjects
Animals
Cell Survival - drug effects
Cercopithecus aethiops
Chromatography
Cytoplasmic Vesicles
Enterotoxins - pharmacology
Exports
INFECTIOUS DISEASES
Lethal cytotoxic enterotoxin (LCE)
Lethal Dose 50
Male
Membrane vesicles (MVs)
Microscopy, Electron, Scanning
Neutralization Tests
Original
Plesiomonas - metabolism
Plesiomonas - pathogenicity
Plesiomonas - ultrastructure
Plesiomonas shigelloides
Rabbits
Rivers - microbiology
Toxin transportation
Vero Cells
Virulence Factors
Lethal cytotoxic enterotoxin (LCE)
Plesiomonas shigelloides
Membrane vesicles (MVs)
Toxin transportation
Virulence factors
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Summary:Plesiomonas shigelloides isolated from water in Brazil was previously described as a hemorrhagic heat-labile cytotoxic-enterotoxin producer. We purified this toxin from culture supernatants using ion metallic affinity chromatography (IMAC) followed by molecular exclusion chromatography. The pure toxin presented molecular mass of 50kDa and isoelectric point (pI) around 6.9 by 2D electrophoresis. When injected intravenously, the purified cytotoxic-enterotoxin induced also severe spasms followed by sudden death of mice. Hence, we entitled it as lethal cytotoxic-enterotoxin (LCE). The presence of membrane vesicles (MVs) on cell surfaces of P. shigelloides was observed by scan electron microscopy (SEM). From these MVs the LCE toxin was extracted and confirmed by biological and serological assays. These data suggest that P. shigelloides also exports this cytotoxic-enterotoxin by membrane vesicles, a different mechanism of delivering extra cellular virulence factors, so far not described in this bacterium.
ISSN:1413-8670
1678-4391
1678-4391
DOI:10.1016/j.bjid.2016.08.014