Cryopreservation of intact human articular cartilage
Damaged articular cartilage (AC) impairs joint function and many treatment techniques are being investigated to determine their long term results. Successful cryopreservation of AC can provide a reliable source of intact matrix with viable chondrocytes to maintain the cartilage over long periods of...
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Published in | Journal of orthopaedic research Vol. 20; no. 6; pp. 1253 - 1255 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Elsevier Ltd
01.11.2002
Wiley Subscription Services, Inc., A Wiley Company Blackwell Publishing Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Damaged articular cartilage (AC) impairs joint function and many treatment techniques are being investigated to determine their long term results. Successful cryopreservation of AC can provide a reliable source of intact matrix with viable chondrocytes to maintain the cartilage over long periods of time. This study investigated the application of an established cryopreservation protocol to determine the recovery of intact chondrocytes from human AC. Ten millimeter diameter osteochondral dowels were harvested from two human donors. The cryopreservation protocol was performed and the samples were rapidly warmed from varying experimental holding temperatures (−10, −20, −30, −40 °C), with and without plunging into liquid nitrogen, using 1 M dimethyl sulfoxide as cryoprotectant. The cartilage was stained with membrane integrity dyes and viewed under fluorescence microscopy. The percent of intact chondrocytes was compared to fresh controls. Low recovery of intact chondrocytes was recorded from all temperature levels with and without cryoprotectant. The results of this experiment demonstrated that the cryopreservation procedure used to achieve moderate success with intact sheep AC was not successful with intact human AC and further investigation is required. |
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Bibliography: | ark:/67375/WNG-6ZSML9P8-5 The Canadian Institute of Health Research, Edmonton Civic Employees istex:D605CD3897ED6FAD4D93420951AD43268B3DBAB2 ArticleID:JOR1100200619 Division of Orthopaedic Surgery of the University of Alberta ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0736-0266 1554-527X |
DOI: | 10.1016/S0736-0266(02)00061-X |