Science Letters： A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana

• Published in: Journal of Zhejiang University. B. Science Vol. 8; no. 4; pp. 256 - 259
• Main Author: CHEN Bing JIANG Jin-hua ZHOU Xue-ping
• Format: Journal Article
• Language: English
• Published: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China 2007; State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China%State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
• Subjects: NbTOM1; 本氏烟; 烟草花叶病毒; 病毒诱发基因沉默; Nicotiana benthamiana; Tobacco mosaic virus; NbTOM1; Virus induced gene silencing
• ISSN: 1673-1581; 1862-1783

The AtTOM1 gene ofArabidopsis thaliana had been shown to be essential for the efficient multiplication of Tobacco mosaic virus （TMV） in A. thaliana. In this study, we cloned an AtTOM1-like gene from Nicotiana benthamiana named as NbTOM1. Sequence alignment showed that NbTOM1 is closely related to AtTOM1 homologues of N. tabacum and Lycopersicon esculentum with 97.2% and 92.6% nucleotide sequence identities, respectively. Silencing of NbTOM1 by a modified viral satellite DNA-based vector resulted in complete inhibition of the multiplication of TMV in N. benthamiana. The result suggests that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants.