Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

• Published in: PloS one Vol. 11; no. 11; p. e0166705
• Main Authors: Keshri, Gaurav K., Gupta, Asheesh, Yadav, Anju, Sharma, Sanjeev K., Singh, Shashi Bala
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 18.11.2016; Public Library of Science (PLoS)
• Subjects: Actin; Adenosine Triphosphate - metabolism; Angiogenesis; Animals; Biological effects; Biology and Life Sciences; Biomarkers; Biopsy; Continuous radiation; Contraction; Cytochrome; Deoxyribonucleic acid; DNA; Engineering and Technology; Extracellular matrix; Fibroblast growth factor receptors; Fibronectin; Fibronectins; Gene therapy; Glucocorticoids; Healing; Heat shock proteins; Hydrocortisone; I.R. radiation; Immunocompromised Host; Inflammation; Infrared lasers; Injuries; Irradiation; Laser Therapy; Lasers; Light; Light therapy; Matrix Metalloproteinases - metabolism; Medicine and Health Sciences; Muscle proteins; NF-κB protein; Pain; Physiology; Pneumoviridae; Rats; Research and Analysis Methods; Restoration; Skin; Skin - pathology; Studies; Transforming growth factors; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α; Vascular endothelial growth factor; Vascularization; Wound care; Wound healing; Wound Healing - immunology; Wound Healing - radiation effects; India
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0166705

Chronic non-healing cutaneous wounds are often vulnerable in one or more repair phases that prevent normal healing and pose challenges to the use of conventional wound care modalities. In immunosuppressed subject, the sequential stages of healing get hampered, which may be the consequences of dysregulated or stagnant wound inflammation. Photobiomodulation (PBM) or low-level laser (light) therapy (LLLT) emerges as a promising drug-free, non-invasive biophysical approach for promoting wound healing, reduction of inflammation, pain and restoration of functions. The present study was therefore undertaken to evaluate the photobiomodulatory effects of 810 nm diode laser (40 mW/cm2; 22.6 J/cm2) with pulsed (10 and 100 Hz, 50% duty cycle) and continuous wave on full-thickness excision-type dermal wound healing in hydrocortisone-induced immunosuppressed rats. Results clearly delineated that 810 nm PBM at 10 Hz was more effective over continuous and 100 Hz frequency in accelerating wound healing by attenuating the pro-inflammatory markers (NF-kB, TNF-α), augmenting wound contraction (α-SM actin), enhancing cellular proliferation, ECM deposition, neovascularization (HIF-1α, VEGF), re-epithelialization along with up-regulated protein expression of FGFR-1, Fibronectin, HSP-90 and TGF-β2 as compared to the non-irradiated controls. Additionally, 810 nm laser irradiation significantly increased CCO activity and cellular ATP contents. Overall, the findings from this study might broaden the current biological mechanism that could be responsible for photobiomodulatory effect mediated through pulsed NIR 810 nm laser (10 Hz) for promoting dermal wound healing in immunosuppressed subjects.