Expression of serum amyloid A4 in human trophoblast-like choriocarcinoma cell lines and human first trimester/term trophoblast cells

• Published in: Placenta (Eastbourne) Vol. 35; no. 8; pp. 661 - 664
• Main Authors: Rossmann, C, Hammer, A, Koyani, C.N, Kovacevic, A, Siwetz, M, Desoye, G, Poehlmann, T.G, Markert, U.R, Huppertz, B, Sattler, W, Malle, E
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.08.2014; Elsevier; W.B. Saunders
• Subjects: Biological and medical sciences; Cell Line, Tumor; Cytokine; Embryology: invertebrates and vertebrates. Teratology; Female; Fundamental and applied biological sciences. Psychology; Humans; Internal Medicine; Invasion; Obstetrics and Gynecology; Outgrowing extravillous trophoblast; Placenta; Pregnancy; Pregnancy Trimester, First; Pregnancy Trimester, Third; SAA4; Serum Amyloid A Protein - metabolism; Short Communication; Trophoblasts - metabolism; Placenta; Outgrowing extravillous trophoblast; Invasion; SAA4; Cytokine; Human; Trophoblaste; Fetal membrane; First trimester; Malignant tumor; Choriocarcinoma; Pregnancy; Vertebrata; Cell line; Mammalia; Serum; Amyloid; Cell; Cancer
• ISSN: 0143-4004; 1532-3102
• DOI: 10.1016/j.placenta.2014.05.012

Abstract Trophoblast invasion into uterine tissues represents a hallmark of first trimester placental development. As expression of serum amyloid A4 (SAA4) occurs in tumorigenic and invasive tissues we here investigated whether SAA4 is present in trophoblast-like human AC1-M59/Jeg-3 cells and trophoblast preparations of human first trimester and term placenta. SAA4 mRNA was expressed in non-stimulated and cytokine-treated AC1-M59/Jeg-3 cells. In purified trophoblast cells SAA4 mRNA expression was upregulated at weeks 10 and 12 of pregnancy. Western-blot and immunohistochemical staining of first trimester placental tissue revealed pronounced SAA4 expression in invasive trophoblast cells indicating a potential role of SAA4 during invasion.