P53 protein in proliferation, repair and apoptosis of cells

• Published in: Protoplasma Vol. 251; no. 3; pp. 525 - 533
• Main Authors: Wawryk-Gawda, Ewelina, Chylińska-Wrzos, Patrycja, Lis-Sochocka, Marta, Chłapek, Katarzyna, Bulak, Kamila, Jędrych, Marian, Jodłowska-Jędrych, Barbara
• Format: Journal Article
• Language: English
• Published: Vienna Springer-Verlag 01.05.2014; Springer Vienna; Springer Nature B.V
• Subjects: angiogenesis; Animals; apoptosis; Apoptosis - drug effects; Apoptosis - physiology; Biomedical and Life Sciences; Cell Biology; Cell Proliferation - drug effects; Cell Proliferation - physiology; Cladribine - pharmacology; DNA; epithelial cells; Epithelial Cells - cytology; Epithelial Cells - drug effects; Female; genome; Life Sciences; Original; Original Article; Plant Sciences; protein synthesis; Random Allocation; Rats; Rats, Wistar; senescence; Tumor Suppressor Protein p53 - physiology; Up-Regulation; Uterus - cytology; Uterus - embryology; Zoology; Proliferation; p53 protein; Cladribine; Apoptosis
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-013-0548-1

The p53 protein is an important factor of many intra- and extracellular processes. This protein regulates the repair of cellular DNA and induces apoptosis. It is also responsible for the regulation of the senescence and the cell entering the subsequent stages of the cellular cycle. The protein p53 is also involved in inhibiting angiogenesis and the induction of oxidative shock. In our study, we examined the activity of p53 protein in the uterine epithelial cells in rats treated with cladribine. Its action is mainly based on apoptosis induction. We compared the activity of p53 protein in cells with a high apoptosis index and in cells with active repair mechanisms and high proliferation index. We observed stronger p53 protein expression in the epithelial cells of the materials taken 24 h after the last dose of 2-CdA associated with the active process of apoptosis and inhibition of proliferation. After 4 weeks from the last dose of cladribine, the stronger expression of p53 protein was associated with both the existing changes in the cell's genome, the effects of the ongoing repair mechanisms, as well as the high proliferation activity.