Interploid and intraploid hybridizations to produce polyploid Haskap (Lonicera caerulea var. emphyllocalyx) plants

• Published in: Euphytica Vol. 201; no. 1; pp. 15 - 27
• Main Authors: Miyashita, Tomomi, Hoshino, Yoichiro
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.01.2015; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: aneuploidy; Biomedical and Life Sciences; Biotechnology; chromosomes; Colchicine; crossing; diploidy; Flowers & plants; fruits; hexaploidy; Hybridization; Life Sciences; Lonicera; Lonicera caerulea; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Polyploidy; Povidone; seeds; tetraploidy; triploidy; Haskap; Caprifoliaceae; Interploid cross; Intraploid cross; Polyploid
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-014-1159-4

We produced polyploid Haskap (Lonicera caerulea var. emphyllocalyx) plants by performing interploid and intraploid crosses of wild accessions. Embryo rescue in a tetraploid (4x) × diploid (2x) cross produced triploid plants; reciprocal 2x × 4x cross failed to produce viable seeds. Intraploidy crosses of 4x × 4x produced mostly tetraploids but also several hexaploid (6x) and octoploid (8x) plants. Using hexaploids obtained from this cross, we examined reciprocal 4x–6x crosses and found that both produced pentaploid plants. An octoploid was produced by applying colchicine to a tetraploid; a 4x × 8x cross using this plant and aided by embryo rescue culture produced three hexaploid plants, with an aneuploid number of chromosomes. Several plants obtained in this study flowered and set fruits. We discuss the overall efficiency of producing polyploid plants in interploid and intraploid crosses.