Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal

• Published in: Parasites & vectors Vol. 14; no. 1; p. 163
• Main Authors: Dordio, Ana Mafalda, Beck, Relja, Nunes, Telmo, Pereira da Fonseca, Isabel, Gomes, Jacinto
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 18.03.2021; BioMed Central; BMC
• Subjects: Anaplasma; Anemia; Anorexia; Arthropods; Babesia; Bacteria; Blood tests; Canine vector-borne diseases (CVBD); Diagnosis; Disease; Diseases; DNA sequences; DNA sequencing; Dogs; Domestic animals; Ehrlichia; Fever; Health care; Hepatozoon; Identification methods; Infections; Infectious diseases; Light microscopy; Microscopy; Molecular diagnostic techniques; Molecular methods; Mycoplasma; Nucleotide sequence; Optical microscopy; Pathogens; PCR; Polymerase chain reaction; Protozoa; Serology; Southern Portugal; Statistics; Surveying; Thrombocytopenia; Vector-borne diseases; Vectors (Biology); Veterinarians; Veterinary medicine; Veterinary surgeons; Portugal; Europe; Southern Portugal; Molecular methods; Canine vector-borne diseases (CVBD); Dogs
• ISSN: 1756-3305; 1756-3305
• DOI: 10.1186/s13071-021-04650-4

Canine vector-borne diseases (CVBDs) are caused by a wide range of pathogens transmitted by arthropods. They have been an issue of growing importance in recent years; however, there is limited information about the vector-borne pathogens circulating in Portugal. The aim of the present study was to detect canine vector-borne bacteria and protozoa of veterinary and zoonotic importance using molecular methods. One hundred and forty-two dogs from Lisbon, southern Portugal, were tested: 48 dogs from a veterinary hospital clinically suspected of vector-borne diseases and 94 apparently healthy dogs from shelters. Anaplasma spp./Ehrlichia spp., Babesia/Theileria spp., Hepatozoon spp., and Mycoplasma spp. infections were detected by PCR from blood samples and examined under light microscopy. Other information including clinical status and diagnostic test results were collected for each animal. Infections were detected by PCR in 48 (33.80%) dogs. Single infections were found in 35 dogs (24.64%), and co-infections were found in 13 (9.15%) dogs. Twenty-nine (20.42%) dogs were positive for Hepatozoon spp., 15 (10.56%) for Mycoplasma spp., 11 (7.75%) for Anaplasma spp./Ehrlichia spp., and six (4.21%) for Babesia spp. DNA sequencing was used to identify Babesia vogeli (2.81%), Babesia canis (1.40%), Hepatozoon canis (20.42%), Mycoplasma haematoparvum (2.11%), Mycoplasma haemocanis (8.45%), Anaplasma platys (7.04%), and Ehrlichia canis (0.70%). This is the first molecular identification of B. canis and M. haematoparvum in dogs from southern Portugal. This study highlights the importance of molecular methods to identify CVBD pathogens in endemic areas and helps to guide the clinical approach of veterinarians in practice.