Roles of osteopontin and matrix metalloproteinase-7 in occurrence, progression, and prognosis of nonsmall cell lung cancer

• Published in: Journal of research in medical sciences Vol. 20; no. 12; pp. 1138 - 1146
• Main Authors: Sun, Ying, Li, Dan, Lv, Xiao-Hong, Hua, Shu-Cheng, Han, Ji-Chang, Xu, Feng, Li, Xian-Dong
• Format: Journal Article
• Language: English
• Published: India Wolters Kluwer - Medknow Publications 01.12.2015; Medknow Publications & Media Pvt. Ltd; Medknow Publications & Media Pvt Ltd; Wolters Kluwer Medknow Publications
• Subjects: Age; Antigens; Biotechnology industry; Cancer therapies; Cardiovascular disease; Conflicts of interest; Hospitals; Lung cancer; Lymph node metastasis; Lymphatic system; matrix metalloproteinase-7 (MMP-7); Medical prognosis; Metastasis; nonsmall cell lung cancer (NSCLC); Original; osteopontin (OPN); prognosis; Protein expression; Proteins; Roles; Thoracic surgery; tumor node metastasis (TNM) staging; Tumors; Lymph node metastasis; osteopontin (OPN); matrix metalloproteinase-7 (MMP-7); nonsmall cell lung cancer (NSCLC); prognosis; tumor node metastasis (TNM) staging
• ISSN: 1735-1995; 1735-1995; 1735-7136
• DOI: 10.4103/1735-1995.172980

Background: This study detected osteopontin (OPN) and matrix metalloproteinase-7 (MMP-7) expressions to explore the roles of OPN and MMP-7 in the occurrence, progression, and prognosis of nonsmall cell lung cancer (NSCLC). Materials and Methods: A retrospective study was conducted on NSCLC tissues (n = 152; case group) and adjacent nonneoplastic lung parenchyma (adjacent to tumor >5 cm; n = 152; control group) collected from 152 NSCLC patients. The protein expressions of OPN and MMP-7 were detected by immunohistochemistry. OPN and MMP-7 messenger RNA (mRNA) expressions were detected by reverse transcription polymerase chain reaction (RT-PCR). Results: The protein and mRNA expressions of OPN and MMP-7 in NSCLC tissues were evidently higher than those in adjacent nonneoplastic lung parenchyma (all P < 0.05). OPN protein and mRNA expression were associated with the degree of differentiation, tumor node metastasis (TNM) staging, and lymph node metastasis in NSCLC (all P < 0.05). MMP-7 protein expression was associated with TNM staging and lymph node metastasis (both P < 0.05) while MMP-7 mRNA expression was associated with the degree of differentiation, TNM staging, and lymph node metastasis (all P < 0.05). A significantly positive relativity was revealed between OPN expression and MMP-7 expression (protein: r = 0.789, P < 0.001; mRNA: r = 0.377, P < 0.001). Lymph node metastasis, TNM staging, OPN, and MMP-7 protein expressions were independent risk factors for the prognosis of NSCLC (all P < 0.05). Conclusion: High MMP-7 and OPN protein expressions are closely related to the occurrence, progression, and prognosis of NSCLC, and can be served as unfavorable prognostic factors for NSCLC.