PS1 activates PI3K thus inhibiting GSK-3 activity and tau overphosphorylation: effects of FAD mutations

• Published in: The EMBO journal Vol. 23; no. 13; pp. 2586 - 2596
• Main Authors: Baki, Lia, Shioi, Junichi, Wen, Paul, Shao, Zhiping, Schwarzman, Alexander, Gama-Sosa, Miguel, Neve, Rachael, Robakis, Nikolaos K
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 07.07.2004; Blackwell Publishing Ltd; Nature Publishing Group
• Subjects: Alzheimer Disease - genetics; Alzheimer's disease; Animals; Apoptosis; Blotting, Western; cadherin; Cadherins - metabolism; Carbamates - pharmacology; Cell Line, Transformed; Cell Survival; Cell Transformation, Viral; Dipeptides - pharmacology; Enzyme Activation; Enzyme Inhibitors - pharmacology; Fibroblasts - metabolism; Flow Cytometry; Glycogen Synthase Kinase 3 - antagonists & inhibitors; Humans; Inactivation; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mice - embryology; Mice, Knockout; Mutation; Pathology; Phosphatidylinositol 3-Kinases - chemistry; Phosphatidylinositol 3-Kinases - metabolism; Phosphorylation; PI3K; Precipitin Tests; Presenilin; Presenilin-1; Protein Structure, Tertiary; Protein-Serine-Threonine Kinases - metabolism; Proto-Oncogene Proteins - metabolism; Proto-Oncogene Proteins c-akt; Residues; Simplexvirus - genetics; Survival; tau; tau Proteins - antagonists & inhibitors; Upstream
• ISSN: 0261-4189; 1460-2075
• DOI: 10.1038/sj.emboj.7600251

Phosphatidylinositol 3‐kinase (PI3K) promotes cell survival and communication by activating its downstream effector Akt kinase. Here we show that PS1, a protein involved in familial Alzheimer's disease (FAD), promotes cell survival by activating the PI3K/Akt cell survival signaling. This function of PS1 is unaffected by γ‐secretase inhibitors. Pharmacological and genetic evidence indicates that PS1 acts upstream of Akt, at or before PI3K kinase. PS1 forms complexes with the p85 subunit of PI3K and promotes cadherin/PI3K association. Furthermore, conditions that inhibit this association prevent the PS1‐induced PI3K/Akt activation, indicating that PS1 stimulates PI3K/Akt signaling by promoting cadherin/PI3K association. By activating PI3K/Akt signaling, PS1 promotes phosphorylation/inactivation of glycogen synthase kinase‐3 (GSK‐3), suppresses GSK‐3‐dependent phosphorylation of tau at residues overphosphorylated in AD and prevents apoptosis of confluent cells. PS1 FAD mutations inhibit the PS1‐dependent PI3K/Akt activation, thus promoting GSK‐3 activity and tau overphosphorylation at AD‐related residues. Our data raise the possibility that PS1 may prevent development of AD pathology by activating the PI3K/Akt signaling pathway. In contrast, FAD mutations may promote AD pathology by inhibiting this pathway.