Neutralizing antibodies for orthobunyaviruses in Pantanal, Brazil

• Published in: PLoS neglected tropical diseases Vol. 11; no. 11; p. e0006014
• Main Authors: Pauvolid-Corrêa, Alex, Campos, Zilca, Soares, Raquel, Nogueira, Rita Maria Ribeiro, Komar, Nicholas
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.11.2017; Public Library of Science (PLoS)
• Subjects: Alligators and Crocodiles - virology; Animals; Animals, Domestic - virology; Animals, Wild - virology; Antibodies; Antibodies, Neutralizing - blood; Antibodies, Viral - blood; Antigen-antibody reactions; Arboviruses; Arthropods; Biology and Life Sciences; Brazil - epidemiology; Bunyaviridae Infections - epidemiology; Bunyaviridae Infections - immunology; Bunyaviridae Infections - veterinary; Bunyaviridae Infections - virology; Detection; Disease control; Disease hot spots; Distribution; Domestic animals; Environmental aspects; Epidemiology; Funding; Horse Diseases - epidemiology; Horse Diseases - immunology; Horse Diseases - virology; Horses - virology; Immunoglobulins; Infections; Medicine and Health Sciences; Neutralization; Orthobunyavirus; Orthobunyavirus - immunology; Orthobunyavirus - isolation & purification; People and places; Physiological aspects; Ranches; Reduction; Reptiles & amphibians; Seroepidemiologic Studies; Sheep; Sheep - virology; Sheep Diseases - epidemiology; Sheep Diseases - immunology; Sheep Diseases - virology; Studies; Tropical diseases; Vertebrates; Viruses; Wild animals; Brazil; United States; United States > US
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0006014

The Pantanal is a hotspot for arbovirus studies in South America. Various medically important flaviviruses and alphaviruses have been reported in domestic and wild animals in the region. To expand the knowledge of local arbovirus circulation, a serosurvey for 14 Brazilian orthobunyaviruses was conducted with equines, sheep and free-ranging caimans. Sera were tested for specific viral antibodies using plaque-reduction neutralization test (PRNT). Monotypic reactions were detected for Maguari, Xingu, Apeu, Guaroa, Murutucu, Oriboca, Oropouche and Nepuyo viruses. Despite the low titers for most of the orthobunyaviruses tested, the detection of monotypic reactions for eight orthobunyaviruses suggests the Pantanal as a region of great orthobunyavirus diversity. The present data, in conjunction with previous studies that detected a high diversity of other arboviruses, ratify the Pantanal as an important natural reservoir for sylvatic and medically important arboviruses in Brazil.