Target deletion of the cytoskeleton-associated protein palladin does not impair neurite outgrowth in mice

• Published in: PloS one Vol. 4; no. 9; p. e6916
• Main Authors: Shu, Run-Zhe, Zhang, Feng, Liu, Xue-Song, Li, Chun-Liang, Wang, Long, Tai, Yi-Lin, Wu, Xiao-Lin, Yang, Xue, Liao, Xiao-Dong, Jin, Ying, Gu, Ming-Min, Huang, Lei, Pang, Xiao-Fen, Wang, Zhu-Gang
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 04.09.2009; Public Library of Science (PLoS)
• Subjects: Actin; Animals; Axonogenesis; Biology; Brain; Brain - embryology; Brain research; Cell adhesion & migration; Cell Biology/Cell Adhesion; Cell Biology/Cytoskeleton; Cell Proliferation; Clonal deletion; Cytoskeletal Proteins - metabolism; Cytoskeleton; Cytoskeleton - metabolism; Defects; Embryos; Extracellular matrix; Gene Expression Regulation, Developmental; Growth cones; Health sciences; Immunoglobulins; Innervation; Isoforms; Laboratories; Lethality; Medicine; Mice; Mice, Knockout; Models, Biological; Morphogenesis; Nervous system; Nervous System - embryology; Neural stem cells; Neural tube; Neurites - metabolism; Neurons; Neurons - metabolism; Neuroscience/Neurodevelopment; Neuroscience/Neuronal and Glial Cell Biology; Phosphoproteins - metabolism; Protein Isoforms; Proteins; Rodents; Skull; Stem cells; Stem Cells - metabolism; Systems development; Time Factors; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0006916

Palladin is an actin cytoskeleton-associated protein which is crucial for cell morphogenesis and motility. Previous studies have shown that palladin is localized to the axonal growth cone in neurons and may play an important role in axonal extension. Previously, we have generated palladin knockout mice which display cranial neural tube closure defect and embryonic lethality before embryonic day 15.5 (E15.5). To further study the role of palladin in the developing nervous system, we examined the innervation of palladin-deficient mouse embryos since the 200 kd, 140 kd, 90-92 kd and 50 kd palladin isoforms were undetectable in the mutant mouse embryo brain. Contrary to the results of previous studies, we found no inhibition of the axonal extension in palladin-deficient mouse embryos. The cortical neurons derived from palladin-deficient mice also showed no significant difference in neurite outgrowth as compared with those from wild-type mice. Moreover, no difference was found in neurite outgrowth of neural stem cell derived-neurons between palladin-deficient mice and wild-type mice. In conclusion, these results suggest that palladin is dispensable for normal neurite outgrowth in mice.