M2 Macrophage‐Derived sEV Regulate Pro‐Inflammatory CCR2+ Macrophage Subpopulations to Favor Post‐AMI Cardiac Repair

• Published in: Advanced science Vol. 10; no. 14; pp. e2202964 - n/a
• Main Authors: Li, Lan, Cao, Jiasong, Li, Sheng, Cui, Tianyi, Ni, Jingyu, Zhang, Han, Zhu, Yan, Mao, Jingyuan, Gao, Xiumei, Midgley, Adam C., Zhu, Meifeng, Fan, Guanwei
• Format: Journal Article
• Language: English
• Published: Germany John Wiley & Sons, Inc 01.05.2023; John Wiley and Sons Inc; Wiley
• Subjects: Animals; Anti-Inflammatory Agents - therapeutic use; CC chemokine receptor 2; Enzymes; Extracellular vesicles; Heart attacks; Heart failure; Inflammation; Ischemia; ischemia‐reperfusion injury; Kinases; macrophage metabolic reprogramming; macrophages; Macrophages - physiology; MicroRNAs; Myocardial Infarction - drug therapy; Rats; Receptors, CCR2 - genetics; Swine; ischemia-reperfusion injury; macrophages; extracellular vesicles; CC chemokine receptor 2; macrophage metabolic reprogramming
• ISSN: 2198-3844; 2198-3844
• DOI: 10.1002/advs.202202964

Tissue‐resident cardiac macrophage subsets mediate cardiac tissue inflammation and repair after acute myocardial infarction (AMI). CC chemokine receptor 2 (CCR2)‐expressing macrophages have phenotypical similarities to M1‐polarized macrophages, are pro‐inflammatory, and recruit CCR2+ circulating monocytes to infarcted myocardium. Small extracellular vesicles (sEV) from CCR2̶ macrophages, which phenotypically resemble M2‐polarized macrophages, promote anti‐inflammatory activity and cardiac repair. Here, the authors harvested M2 macrophage‐derived sEV (M2EV) from M2‐polarized bone‐marrow‐derived macrophages for intramyocardial injection and recapitulation of sEV‐mediated anti‐inflammatory activity in ischemic‐reperfusion (I/R) injured hearts. Rats and pigs received sham surgery; I/R without treatment; or I/R with autologous M2EV treatment. M2EV rescued cardiac function and attenuated injury markers, infarct size, and scar size. M2EV inhibited CCR2+ macrophage numbers, reduced monocyte‐derived CCR2+ macrophage recruitment to infarct sites, induced M1‐to‐M2 macrophage switching and promoted neovascularization. Analysis of M2EV microRNA content revealed abundant miR‐181b‐5p, which regulated macrophage glucose uptake, glycolysis, and mitigated mitochondrial reactive oxygen species generation. Functional blockade of miR‐181b‐5p is detrimental to beneficial M2EV actions and resulted in failure to inhibit CCR2+ macrophage numbers and infarct size. Taken together, this investigation showed that M2EV rescued myocardial function, improved myocardial repair, and regulated CCR2+ macrophages via miR‐181b‐5p‐dependent mechanisms, indicating an option for cell‐free therapy for AMI. M2 macrophage‐derived small extracellular vesicles (M2EV) regulated metabolic reprogramming of CC chemokine receptor 2 (CCR2)+ macrophages by delivery of miR‐181b‐5p, which in turn prevented the CCR2+ > CCR2− macrophage imbalance observed after ischemic‐reperfusion (I/R) injury induced myocardial infarction. This results in the inhibition of the recruitment of CCR2+ monocytes and monocyte‐derived macrophages to the myocardium, thereby promoting cardiac tissue repair.