Development of magnesium calcium phosphate biocement for bone regeneration

• Published in: Journal of the Royal Society interface Vol. 7; no. 49; pp. 1171 - 1180
• Main Authors: Jia, Junfeng, Zhou, Huanjun, Wei, Jie, Jiang, Xin, Hua, Hong, Chen, Fangping, Wei, Shicheng, Shin, Jung-Woog, Liu, Changsheng
• Format: Journal Article
• Language: English
• Published: England The Royal Society 06.08.2010
• Subjects: Biocement; Biocompatibility; Bone Regeneration - physiology; Calcium - chemistry; Calcium Phosphate; Calcium Phosphates - chemistry; Calcium, Dietary; Compressive Strength; Degradation; Humans; Magnesium; Magnesium Compounds; Magnesium Phosphate; Microscopy, Electron, Scanning; Phosphates - chemistry
• ISSN: 1742-5689; 1742-5662
• DOI: 10.1098/rsif.2009.0559

Magnesium calcium phosphate biocement (MCPB) with rapid-setting characteristics was fabricated by using the mixed powders of magnesium oxide (MgO) and calcium dihydrogen phosphate (Ca(H2PO4)2·H2O). The results revealed that the MCPB hardened after mixing the powders with water for about 7 min, and the compressive strength reached 43 MPa after setting for 1 h, indicating that the MCPB had a short setting time and high initial mechanical strength. After the acid–base reaction of MCPB containing MgO and Ca(H2PO4)2·H2O in a molar ratio of 2 : 1, the final hydrated products were Mg3(PO4)2 and Ca3(PO4)2. The MCPB was degradable in Tris–HCl solution and the degradation ratio was obviously higher than calcium phosphate biocement (CPB) because of its fast dissolution. The attachment and proliferation of the MG63 cells on the MCPB were significantly enhanced in comparison with CPB, and the alkaline phosphatase activity of MG63 cells on the MCPB was significantly higher than on the CPB at 7 and 14 days. The MG63 cells with normal phenotype spread well on the MCPB surfaces, and were attached in close proximity to the substrate, as seen by scanning electron microscopy (SEM). The results demonstrated that the MCPB had a good ability to support cell attachment, proliferation and differentiation, and exhibited good cytocompatibility.