Chronic hyperadiponectinemia induced by transgenic overexpression increases plasma exosomes without significantly improving glucose and lipid metabolism

• Published in: ENDOCRINE JOURNAL Vol. 70; no. 6; pp. 635 - 645
• Main Authors: Kawada, Keitaro, Kita, Shunbun, Fukuda, Shiro, Fukuoka, Keita, Okita, Tomonori, Kawada-Horitani, Emi, Iioka, Masahito, Sakaue, Taka-aki, Kawachi, Yusuke, Fujii, Kohei, Kimura, Yu, Otabe, Shuichi, Fujishima, Yuya, Nishizawa, Hitoshi, Maeda, Norikazu, Shimomura, Iichiro
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Japan Endocrine Society 2023; Japan Science and Technology Agency
• Subjects: Adiponectin; Energy metabolism; Exosomes; Extracellular vesicles; Fat metabolism; Glucose metabolism; High fat diet; Homeostasis; Lipid metabolism; Streptozocin; T-cadherin; Transgenic mice; Extracellular vesicles; Glucose metabolism; Lipid metabolism; Exosomes; Adiponectin
• ISSN: 0918-8959; 1348-4540
• DOI: 10.1507/endocrj.EJ22-0665

The fat-derived factor, adiponectin, is considered a salutary circulating factor. We recently demonstrated that native adiponectin binds T-cadherin and promotes intracellular biogenesis and secretion of the exosome. Exosomes play important roles in various aspects of homeostasis, including glucose and energy metabolism. However, it remains unclear whether and how the promotion of exosome production by adiponectin in vivo is beneficial for glucose and lipid metabolism. In the present study, overexpression of human adiponectin in mice resulted in an increased number of circulating exosomes, but it did not significantly improve glucose metabolism, change body weights, or change triglyceride clearance under a high-fat diet. Multiple small doses of streptozotocin increased blood glucose and decreased triglyceride clearance similarly in both wild-type and transgenic mice. Thus, these results indicated that human adiponectin overexpression in mice increases plasma exosomes but does not significantly influence glucose and lipid metabolism.