Inhibition of cholinesterase by essential oil from food plant

• Published in: Phytomedicine (Stuttgart) Vol. 19; no. 8-9; pp. 836 - 839
• Main Authors: Chaiyana, Wantida, Okonogi, Siriporn
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 15.06.2012; Urban & Fischer Verlag
• Subjects: Acetylcholinesterase; Acetylcholinesterase - metabolism; Alzheimer disease; Alzheimer's disease; Animals; Butyrylcholinesterase; Butyrylcholinesterase - metabolism; Camphor - analysis; Chemical properties; Cholinesterase; Cholinesterase Inhibitors - chemistry; Cholinesterase Inhibitors - pharmacology; Cholinesterases; Cineole; citronellol; Citrus aurantifolia; Citrus aurantiifolia; Citrus aurantiifolia - chemistry; Colorimetry; Cyclohexanols - analysis; Cyclohexenes - analysis; Drug Evaluation, Preclinical - methods; Ellman's method; Essences and essential oils; Essential oil; Essential oils; Food plants; Gas Chromatography-Mass Spectrometry; Health aspects; Inhibitory Concentration 50; Leaves; Lime fruit; Limonene; Melissa - chemistry; Melissa officinalis; Monoterpenes - analysis; monoterpenoids; Neurodegenerative diseases; Oils, Volatile - chemistry; Oils, Volatile - pharmacology; plant fats and oils; Plant Leaves - chemistry; Plants, Edible - chemistry; Terpenes - analysis; Thailand; Essential oil; Acetylcholinesterase; Butyrylcholinesterase; Ellman's method; Citrus aurantifolia
• ISSN: 0944-7113; 1618-095X; 1618-095X
• DOI: 10.1016/j.phymed.2012.03.010

Inhibition of cholinesterase has attracted much attention recently because of its potential for the treatment of Alzheimer's disease. In this work, the anticholinesterase activities of plant oils were investigated using Ellman's colorimetric method. The results indicate that essential oils obtained from Melissa officinalis leaf and Citrus aurantifolia leaf showed high acetylcholinesterase and butyrylcholinesterase co-inhibitory activities. C. aurantifolia leaf oil revealed in this study has an IC50 value on acetylcholinesterase and butyrylcholinesterase of 139±35 and 42±5μg/ml, respectively. GC/MS analysis revealed that the major constituents of C. aurantifolia leaf oil are monoterpenoids including limonene, l-camphor, citronellol, o-cymene and 1,8-cineole.