Anti-inflammatory effects of gomisin N, gomisin J, and schisandrin C isolated from the fruit of Schisandra chinensis

• Published in: Bioscience, biotechnology, and biochemistry Vol. 74; no. 2; pp. 285 - 291
• Main Authors: Oh, S.Y., Chungbuk National Univ., Cheongju (Korea R.), Kim, Y.H, Bae, D.S, Um, B.H, Pan, C.H, Kim, C.Y, Lee, H.J, Lee, J.K
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 2010; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Animals; ANTI-INFLAMMATOIRE; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; ANTIINFLAMMATORY AGENTS; ANTINFLAMATORIOS; Biological and medical sciences; Cell Line; Cyclooctanes - isolation & purification; Cyclooctanes - pharmacology; DRUG PLANTS; Fruit - metabolism; Fundamental and applied biological sciences. Psychology; gomisin J; gomisin N; http://www.fao.org/aos/agrovoc#c_2393; http://www.fao.org/aos/agrovoc#c_36421; http://www.fao.org/aos/agrovoc#c_3856; http://www.fao.org/aos/agrovoc#c_507; INFLAMACION; INFLAMMATION; LIGNANAS; LIGNANE; LIGNANS; Lignans - isolation & purification; Lignans - pharmacology; Macrophages - drug effects; Macrophages - metabolism; Mice; PLANTAS MEDICINALES; PLANTE MEDICINALE; Polycyclic Compounds - isolation & purification; Polycyclic Compounds - pharmacology; Schiandra chinensis; Schisandra; Schisandra - metabolism; schisandrin C; Fruit; schisandrin C; Antiinflammatory agent; gomisin N; Inflammation; Schiandra chinensis; gomisin J
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.90597

Schiandra chinensis is a well-known Chinese traditional medicine for the treatment of hepatic disease. In this study, we investigated whether the nine major compounds of Schiandra chinensis could be applied to suppress lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages (Raw 264.7 cells). Among the nine lignans, three, gomisin J, gomisin N, and schisandrin C, were found to reduce nitric oxide (NO) production from LPS-stimulated Raw 264.7 cells. These three lignans showed low cytotoxic effects in Raw 264.7 cells. Pre-treatment of Raw 264.7 cells with gomisin J, gomisin N, or schisandrin C reduced the expression of mRNA and the secretion of pro-inflammatory cytokines. These inhibitory effects were found to be caused by blockage of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinases 1 and 2 (ERK 1/2), and c-Jun N-terminal kinase (JNK) phosphorylation.