Functional characterization of a newly identified group B Streptococcus pullulanase eliciting antibodies able to prevent alpha-glucans degradation

• Published in: PloS one Vol. 3; no. 11; p. e3787
• Main Authors: Santi, Isabella, Pezzicoli, Alfredo, Bosello, Mattia, Berti, Francesco, Mariani, Massimo, Telford, John L, Grandi, Guido, Soriani, Marco
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.11.2008; Public Library of Science (PLoS)
• Subjects: Amino Acid Sequence; Amino acids; Anchoring; Animals; Antibodies; Antibodies, Bacterial - biosynthesis; Antibodies, Bacterial - blood; Antibody Specificity; Antigens; Bacteria; Base Sequence; Biodegradation; Carbohydrates; Catalysis; Cell culture; Cell walls; Clonal deletion; Cross Reactions; Degradation; Disease; DNA Primers - genetics; DNA, Bacterial - genetics; Enzymes; Fines & penalties; Flow cytometry; Gene deletion; Gene expression; Genes, Bacterial; Genomes; Genomics; Glucan; Glucans; Glucans - metabolism; Glucose; Glycogen; Glycoside Hydrolases - chemistry; Glycoside Hydrolases - genetics; Glycoside Hydrolases - immunology; Glycoside Hydrolases - metabolism; Gram-positive bacteria; Homology; Humans; Immunization; Incubation; Infectious Diseases/Bacterial Infections; Machinery and equipment; Maltose; Metabolism; Microbiology/Cellular Microbiology and Pathogenesis; Microbiology/Immunity to Infections; Molecular Sequence Data; Mutagenesis; Mutation; Mutualism; NMR; Nuclear magnetic resonance; Nucleotide sequence; Polysaccharides; Proteins; Pullulan; Pullulanase; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Recombinant Proteins - metabolism; Saccharides; SAP gene; SAP protein; Sequence Homology, Amino Acid; Starch; Streptococcus; Streptococcus agalactiae - enzymology; Streptococcus agalactiae - genetics; Streptococcus agalactiae - immunology; Streptococcus infections; Streptococcus pneumoniae; Trisaccharides - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0003787

Streptococcal pullulanases have been recently proposed as key components of the metabolic machinery involved in bacterial adaptation to host niches. By sequence analysis of the Group B Streptococcus (GBS) genome we found a novel putative surface exposed protein with pullulanase activity. We named such a protein SAP. The sap gene is highly conserved among GBS strains and homologous genes, such as PulA and SpuA, have been described in other pathogenic streptococci. The SAP protein contains two N-terminal carbohydrate-binding motifs, followed by a catalytic domain and a C-terminal LPXTG cell wall-anchoring domain. In vitro analysis revealed that the recombinant form of SAP is able to degrade alpha-glucan polysaccharides, such as pullulan, glycogen and starch. Moreover, NMR analysis showed that SAP acts as a type I pullulanase. Studies performed on whole bacteria indicated that the presence of alpha-glucan polysaccharides in culture medium up-regulated the expression of SAP on bacterial surface as confirmed by FACS analysis and confocal imaging. Deletion of the sap gene resulted in a reduced capacity of bacteria to grow in medium containing pullulan or glycogen, but not glucose or maltose, confirming the pivotal role of SAP in GBS metabolism of alpha-glucans. As reported for other streptococcal pullulanases, we found specific anti-SAP antibodies in human sera from healthy volunteers. Investigation of the functional role of anti-SAP antibodies revealed that incubation of GBS in the presence of sera from animals immunized with SAP reduced the capacity of the bacterium to degrade pullulan. Of interest, anti-SAP sera, although to a lower extent, also inhibited Group A Streptococcus pullulanase activity. These data open new perspectives on the possibility to use SAP as a potential vaccine component inducing functional cross-reacting antibodies interfering with streptococcal infections.