Establishment of a new canine cell line (CCT) originated from a cutaneous malignant histiocytosis

• Published in: Journal of Veterinary Medical Science Vol. 65; no. 6; pp. 731 - 735
• Main Authors: Sakai, H. (Gifu Univ. (Japan). Faculty of Agriculture), Nakano, H, Yamaguchi, R, Yonemaru, K, Yanai, T, Masegi, T
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.06.2003; Japan Science and Technology Agency
• Subjects: Animals; canine; CELL CULTURE; Cell Culture Techniques; cell line; Cell Line - pathology; CELLS; DOGS; Histiocytic Disorders, Malignant - pathology; Histiocytic Disorders, Malignant - veterinary; Male; MALIGNANT COURSE; malignant histiocytosis; Skin - pathology; Skin Neoplasms - pathology; Skin Neoplasms - veterinary
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.65.731

A new canine cell line, named CCT, was established from the cutaneous malignant histiocytosis in a 4-year-old male Borzoi. CCT proliferated with loose adherence and doubling time was approximately 30 hr. When co-cultured with latex beads, CCT phagocytized beads vigorously. Lysozyme and vimentin were positive by immunostaining, and non-specific esterase and acid phosphatase were positive by cytochemical staining. These features indicated the cells had a histiocytic nature. Furthermore, by subcutaneous injection to nude mice CCT could successfully form tumors with the morphological and immunohistochemical features similar to the original tumor.