Characterization of the mus308 gene in Drosophila melanogaster

• Published in: Genetics (Austin) Vol. 133; no. 1; pp. 87 - 96
• Main Authors: Leonhardt, E.A. (University of California, San Francisco, CA), Henderson, D.S, Rinehart, J.E, Boyd, J.B
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Genetics Soc America 01.01.1993; Genetics Society of America
• Subjects: ADN; AILE; ALAS; ANEMIAS; ANIMALS; ARTHROPODS; BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; BIOLOGICAL MODELS; Chromatin - metabolism; CHROMOSOMAL ABERRATIONS; CHROMOSOME; Chromosome Mapping; CHROMOSOMES; CITOGENETICA; Classical genetics, quantitative genetics, hybrids; CROMOSOMAS; Crosses, Genetic; CYTOGENETIQUE; DIPTERA; DISEASES; DNA; DNA-ASE; DROSOPHILA; DROSOPHILA MELANOGASTER; Drosophila melanogaster - drug effects; Drosophila melanogaster - genetics; Drosophila melanogaster - physiology; Drosophila melanogaster - radiation effects; Embryo, Nonmammalian - metabolism; ENZYMES; ESTERASES; FLIES; FRUIT FLIES; Fundamental and applied biological sciences. Psychology; GENE; GENE MUTATIONS; GENES; Genetics; Genetics of eukaryotes. Biological and molecular evolution; HEMIC DISEASES; Homozygote; HYDROLASES; INSECTS; Invertebrata; INVERTEBRATES; Investigations; LOCI; LOCOMOCION; LOCOMOTION; LOCUS; MAPPING; Mechlorethamine - toxicity; METABOLISM; MUTACION INDUCIDA; MUTAGENE; MUTAGENOS; Mutagens - toxicity; MUTANT; MUTANTES; Mutation; MUTATION PROVOQUEE; MUTATIONS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; Phenotype; PHOSPHODIESTERASES; PROTEINS; Recombination, Genetic; SYMPTOMS 550400 > Genetics; VIABILIDAD; VIABILITE; Chromosomal aberration; Genetic mapping; Embryonic development; Wing; Insecta; Crosslink agent; Chromosome 3; Drosophilidae; Pleiotropy; Sensitivity resistance; Arthropoda; Mutation; Invertebrata; Drosophila melanogaster; Diptera
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1093/genetics/133.1.87

Among the available mutagen-sensitive mutations in Drosophila, those at the mus308 locus are unique in conferring hypersensitivity to DNA cross-linking agents but not to monofunctional agents. Those mutations are also associated with an elevated frequency of chromosomal aberrations, altered DNA metabolism and the modification of a deoxyribonuclease. This spectrum of phenotypes is shared with selected mammalian mutations including Fanconi anemia in humans. In anticipation of the molecular characterization of the mus308 gene, it has been localized cytogenetically to 87C9-87D1,2 on the right arm of chromosome three. Nine new mutant alleles of the gene have been generated by X-ray mutagenesis and one was recovered following hybrid dysgenesis. Characterization of these new alleles has uncovered additional phenotypes of mutations at this locus. Homozygous mus308 flies that have survived moderate mutagen treatment exhibit an altered wing position that is correlated with reduced flight ability and an altered mitochondria morphology. In addition, observations of elevated embryo mortality are potentially explained by an aberrant distribution of nuclear material in early embryos which is similar to that seen in the mutant giant nuclei