Prognostic value of phagocytic activity of neutrophils and monocytes in sepsis. Correlation to CD64 and CD14 antigen expression

• Published in: Clinical and experimental immunology Vol. 154; no. 1; pp. 87 - 97
• Main Authors: Danikas, D.D, Karakantza, M, Theodorou, G.L, Sakellaropoulos, G.C, Gogos, C.A
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.10.2008; Blackwell Publishing Ltd; Blackwell; Blackwell Science Inc
• Subjects: Aged; Analysis of Variance; Analytical, structural and metabolic biochemistry; Bacterial diseases; Bacterial sepsis; Biological and medical sciences; Biomarkers - analysis; Case-Control Studies; CD18 Antigens - analysis; Female; Flow Cytometry; flow cytometry/FACS; Fundamental and applied biological sciences. Psychology; Human bacterial diseases; Humans; Immunophenotyping; infection; infections; Infectious diseases; Male; Medical sciences; Middle Aged; Monocytes - physiology; neutrophils; Neutrophils - physiology; phagocytosis; Phagocytosis - physiology; phenotype/cell markers; Prognosis; Receptors, IgG - analysis; Sepsis - immunology; Sepsis - mortality; Sepsis - pathology; Translational Studies; Flow cytometry; Prognosis; phenotype/cell markers; Monocyte; Granulocyte; Biological marker; FcγRI receptor; Biochemistry; flow cytometry/FACS; Bacteremia; Infection; Sepsis syndrome; Phenotype; Bacteriosis; infections; Neutrophil; Cell; neutrophils; Phagocytosis
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1111/j.1365-2249.2008.03737.x

The role of the phagocytic function of monocytes and neutrophils in sepsis has been poorly investigated. The present study evaluated the impact of the phagocytic activity of neutrophils and monocytes on the outcome of patients with severe sepsis. Thirty-one patients and 30 healthy individuals were enrolled in the study. The phagocytic activity of monocytes and neutrophils was evaluated during 24 h after admission and the results were correlated to the expression of CD64 on neutrophils and monocytes, CD14 antigen on monocytes, the Simplified Acute Physiology Score II and the patients' survival. A reduced phagocytic activity of neutrophils during the first 24 h after admission was a negative predictor for survival. Increased expression of CD64 antigen on polymorphonuclear cells (PMNs) and monocytes was favourably correlated to the patients' survival. In multivariate analysis the phagocytic activity of PMNs was the only independent predictor factor for survival. Patients with PMN phagocytic activity <37% had lower expression of CD64 on monocytes and PMNs and worse outcome, while those with phagocytic activity >37% had higher expression of CD64 on monocytes and PMNs and better outcome. Reduced phagocytic activity of neutrophils may represent a state of neutrophil inactivation similar to that previously described for monocytes during the compensatory anti-inflammatory response.