IL-36 Cytokine Expression and Its Relationship with p38 MAPK and NF-KB Pathways in Psoriasis Vulgaris Skin Lesions

• Published in: 华中科技大学学报（医学英德文版） Vol. 33; no. 4; pp. 594 - 599
• Main Author: Oi HE Hong-xiang CHEN Wen LI Yan WU Shanguan CHEN Qing YUE Min XIAO Jia-wen Li
• Format: Journal Article
• Language: English
• Published: Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2013
• Subjects: MAPK; NF-κB; p38丝裂原活化蛋白激酶; Western印迹; 因子表; 定量RT-PCR; 白细胞介素; 银屑病; interleukin-36; nuclear factor-kappa B; p38 mitogen-activated protein kinase; psoriasis vulgaris
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-013-1164-1

Summary： This study examined the correlation of the expression of interleukin-36 （IL-36）, a novel member of interleukin-1 （IL-1） family, with p38 mitogen-activated protein kinase （p38 MAPK） and nu clear factor-kappa B （NF-kB） pathways in psoriasis vulgaris skin lesions. The expression levels of IL-36a, IL-3613, IL-367, phosphorylated p38 MAPK, and NF-id3p65 were detected in the skin tissues of 38 psoriasis patients and 17 healthy control subjects by real-time quantitative reverse transcription po lymerase chain reaction （qRT-PCR） and Western blotting. The cytokine expression levels were com pared between the psoriasis group and the control group. A correlation analysis between cytokine pro teins was performed in the psoriasis group. Results showed that the expression levels of IL-36a, IL-3613, IL-36y, phosphorylated p38 MAPK and NF-rh3p65 in the psoriasis group were Significantly higher than those in the control group （P〈0.001）. In the psoriasis group, the IL-36 cytokine expression was positively correlated with phosphorylated p38 MAPK and NF-kBp65 expression （P〈0.05）. A significant positive correlation was also found between the phosphorylated p38 MAPK and NF-v,.Bp65 expression （P〈0.01）. It was concluded that the increased IL-36 expression is correlated with p38 MAPK and NF-kB pathways in psoriasis vulgaris skin lesions. All the three factors may be jointly involved in the pathogenesis and local inflammatory response of psoriasis.