Cloning and Characterization of a Lentinula edodes Class II Chitin Synthase Gene, LeChs2

• Published in: Bioscience, biotechnology, and biochemistry Vol. 74; no. 8; pp. 1707 - 1709
• Main Authors: SATO, Toshitsugu, OKAWA, Kumiko, HIRANO, Tatsuya
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 2010; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Amino acids; Basidiomycetes; Biochemistry; Biological and medical sciences; Biotechnology; Blotting, Northern; Chitin; Chitin Synthase - classification; Chitin Synthase - genetics; chitin synthase gene; class II chitin synthase; Cloning; Cloning, Molecular; Deoxyribonucleic acid; Fundamental and applied biological sciences. Psychology; gene cloning; Genes; Lentinula edodes; Molecular Sequence Data; Phylogeny; Residues; Shiitake Mushrooms - enzymology; Shiitake Mushrooms - genetics; Characterization; class II chitin synthase; gene cloning; Gene; Enzyme; Transferases; Glycosyltransferases; Hexosyltransferases; chitin synthase gene; Chitin synthase; Molecular cloning; Lentinula edodes
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.100222

A genomic DNA sequence and cDNA encoding a putative chitin synthase were isolated from the white rot basidiomycete Lentinula edodes. The gene, named LeChs2, consists of a 2,598-bp open reading frame interrupted by 14 introns and encodes a putative protein of 866 amino acid residues. The data obtained in this study suggest that LeChs2 belongs to the class II chitin synthases.