The role of phosphoinositide 3-kinase subunits in chronic thyroiditis

• Published in: Thyroid research Vol. 5; no. 1; p. 22
• Main Authors: Wojciechowska-Durczynska, Katarzyna, Krawczyk-Rusiecka, Kinga, Cyniak-Magierska, Anna, Zygmunt, Arkadiusz, Sporny, Stanisław, Lewinski, Andrzej
• Format: Journal Article
• Language: English
• Published: London BioMed Central 21.12.2012; BioMed Central Ltd; BMC
• Subjects: Analysis; B cells; Cancer; Cancer Research; Cellular signal transduction; Chronic thyroiditis; Detectors; Endocrinology; Gene expression; Genes; Genetic aspects; Medical research; Medicine; Medicine & Public Health; Metabolic disorders; Oncology; Phosphoinositides; Physiological aspects; PI3K isoforms; Studies; Thyroid cancer; Thyroid gland; Thyroiditis; PI3K isoforms; Chronic thyroiditis
• ISSN: 1756-6614; 1756-6614
• DOI: 10.1186/1756-6614-5-22

Background The risk of neoplastic transformation in patients with chronic thyroiditis (Hashimoto’s thyroiditis – HT) is slightly increased. Genetic background of this observation is still unclear. PI3K isoforms are linked with inflammatory and neoplastic processes, thus they appear to be interesting subjects of a research in this respect. The aim of our study was to assess the PIK3CA , PIK3CB , PIK3CD and PIK3CG genes expression levels in HT. Methods Following conventional cytological examination, 67 thyroid FNAB specimens, received from patients with HT, were quantitatively evaluated regarding PIK3CA , PIK3CB , PIK3CD and PIK3CG expression levels by real-time PCR in the ABI PRISM ® 7500 Sequence Detection System. Results The performed analysis has revealed significantly higher expression levels (RQ) of PIK3CD , PIK3CG and PIK3CA genes in comparison with PIK3CB gene (p<0.05) and significantly higher gene expression level of PIK3CD in comparison with PIK3CA (p<0.05). Conclusion The observed increased PIK3CD , PIK3CG genes expression in HT is probably related to lymphocyte infiltration commonly seen in this condition, however, the role of increased PIK3CA gene expression in the multi-step carcinogenesis process cannot be excluded.