Transcriptomic dissection reveals wide spread differential expression in chickpea during early time points of Fusarium oxysporum f. sp. ciceri Race 1 attack

• Published in: PloS one Vol. 12; no. 5; p. e0178164
• Main Authors: Gupta, Sumanti, Bhar, Anirban, Chatterjee, Moniya, Ghosh, Amartya, Das, Sampa
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25.05.2017; Public Library of Science (PLoS)
• Subjects: Abscisic acid; Actin; Adapters; Alfalfa; Amplified fragment length polymorphism; Anchoring; Aquaporins; Arabinogalactan; Assembly; Barley; Biochemistry; Biology; Biology and life sciences; Biotechnology; Botany; Breakdown; Breeding; Chemical attack; Chick peas; Cicer - metabolism; Cicer - microbiology; Computer and Information Sciences; Cortex; Crops; Damage; Defects; Diseases and pests; Dissection; Environmental conditions; Fungicides; Fusarium; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant - physiology; Gene mapping; Gene Ontology; Genetic aspects; Genomes; Iron; Isoenzymes; Legumes; Mathematical analysis; Membranes; Metabolism; Microtubules; Nitric oxide; Nitrogen; Nodules; Nucleotide sequence; Nutritive value; Osmoregulation; Oxygen; Pathogens; Physiological aspects; Plant biology; Plant Diseases - microbiology; Plant sciences; Plastids; Proteins; Radar; Reactive oxygen species; Real-Time Polymerase Chain Reaction; Regulators; Research and Analysis Methods; Ribonucleic acid; RNA; Roots; Sugar; Tomatoes; Transcription factors; Water deficit; Wounds; India; West Bengal India
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0178164

Plants' reaction to underground microorganisms is complex as sessile nature of plants compels them to prioritize their responses to diverse microorganisms both pathogenic and symbiotic. Roots of important crops are directly exposed to diverse microorganisms, but investigations involving root pathogens are significantly less. Thus, more studies involving root pathogens and their target crops are necessitated to enrich the understanding of underground interactions. Present study reported the molecular complexities in chickpea during Fusarium oxysporum f. sp. ciceri Race 1 (Foc1) infection. Transcriptomic dissections using RNA-seq showed significantly differential expression of molecular transcripts between infected and control plants of both susceptible and resistant genotypes. Radar plot analyses showed maximum expressional undulations after infection in both susceptible and resistant plants. Gene ontology and functional clustering showed large number of transcripts controlling basic metabolism of plants. Network analyses demonstrated defense components like peptidyl cis/trans isomerase, MAP kinase, beta 1,3 glucanase, serine threonine kinase, patatin like protein, lactolylglutathione lyase, coproporphyrinogen III oxidase, sulfotransferases; reactive oxygen species regulating components like respiratory burst oxidase, superoxide dismutases, cytochrome b5 reductase, glutathione reductase, thioredoxin reductase, ATPase; metabolism regulating components, myo inositol phosphate, carboxylate synthase; transport related gamma tonoplast intrinsic protein, and structural component, ubiquitins to serve as important nodals of defense signaling network. These nodal molecules probably served as hub controllers of defense signaling. Functional characterization of these hub molecules would not only help in developing better understanding of chickpea-Foc1 interaction but also place them as promising candidates for resistance management programs against vascular wilt of legumes.